{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T06:06:06Z","timestamp":1762927566981},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[1999,6,1]],"date-time":"1999-06-01T00:00:00Z","timestamp":928195200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,6]]},"abstract":"<jats:p>A protein phosphatase exclusively dependent upon micromolar amounts of Ca<jats:sup>2+<\/jats:sup> and calmodulin has been identified and \npartially purified from <jats:italic>Leishmania<\/jats:italic> spp. Complete obliteration of its activity is observed in the presence of calmodulin \nantagonists such as trifluoperazine, fluphenazine and calmidazolium. Relative insensitivity to okadaic acid and lack of \nactivation in the absence of Ca<jats:sup>2+<\/jats:sup> and calmodulin distinguishes this enzyme from PP1, PP2A and PP2C-type protein \nphosphatases. Cross-reactivity of the enzyme was observed with antibodies that recognize both the A and B chains of \ncalcineurin, a PP2B type Ca<jats:sup>2+<\/jats:sup> and calmodulin-dependent phosphatase from brain. FK506, an immunosuppresive drug \nthat inhibits the enzyme from other sources inhibited the enzyme only in the presence of exogenous FK binding protein, \nwhereas Cyclosporin A inhibited the enzyme in crude preparations. Taken together these results reveal the presence of \na Ca<jats:sup>2+<\/jats:sup> and calmodulin-dependent phosphatase from <jats:italic>Leishmania<\/jats:italic>. This is the first report of the presence of a PP2B-type \nprotein phosphatase from a pathogenic protozoa.<\/jats:p>","DOI":"10.1017\/s0031182099004308","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"567-573","source":"Crossref","is-referenced-by-count":24,"title":["Ca<sup>2+<\/sup> and calmodulin-dependent protein phosphatase from \n<i>Leishmania donovani<\/i>"],"prefix":"10.1017","volume":"118","author":[{"given":"C.","family":"BANERJEE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"SARKAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"BHADURI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,6,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099004308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T20:01:54Z","timestamp":1555012914000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099004308\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,6]]}},"alternative-id":["S0031182099004308"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099004308","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,6]]}}}