{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T14:42:06Z","timestamp":1697467326140},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1999,8,1]],"date-time":"1999-08-01T00:00:00Z","timestamp":933465600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:p>A cDNA library constructed from 3 day post-infective L3 of the filarial nematode <jats:italic>Brugia pahangi<\/jats:italic> was screened by \ndifferential hybridization with cDNA probes prepared from different life-cycle stages. Five cDNA clones hybridizing \nselectively to the mosquito-derived L3 probe were isolated and characterized. Northern blot analysis of 4 of the clones \nconfirmed that each was most highly expressed in the mosquito-derived L3. The expression of each mRNAduring parasite \ndevelopment in the mosquito vector was investigated using RT\u2013PCR, and all were shown to be abundant in the immature \nL3. Four of the 5 cDNAs cloned coded for structural proteins: 2 cuticular collagens, and the muscle proteins tropomyosin \nand troponin. Further studies on troponin using an antiserum raised to the recombinant protein demonstrated that the \nprotein, unlike the mRNA, was present in all life-cycle stages examined, while immunogold labelling demonstrated that \nit was localized to the muscle blocks.<\/jats:p>","DOI":"10.1017\/s0031182099004576","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"189-198","source":"Crossref","is-referenced-by-count":12,"title":["The isolation of differentially expressed cDNA clones \nfrom the filarial nematode <i>Brugia pahangi<\/i>"],"prefix":"10.1017","volume":"119","author":[{"given":"S. J.","family":"HUNTER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. A. M.","family":"MARTIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"THOMPSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"TETLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"DEVANEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,8,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099004576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,7]],"date-time":"2019-04-07T15:21:40Z","timestamp":1554650500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099004576\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,8]]}},"alternative-id":["S0031182099004576"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099004576","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,8]]}}}