{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T21:37:22Z","timestamp":1648762642709},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1999,9,1]],"date-time":"1999-09-01T00:00:00Z","timestamp":936144000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:p>Localization of the type 2 <jats:italic>Alu<\/jats:italic> sequence (B2), a highly repetitive DNA sequence in the mouse genome, was examined by \n<jats:italic>in situ<\/jats:italic> polymerase chain reaction (<jats:italic>in situ<\/jats:italic> PCR) in schistosomes. The signals to the B2 sequence were detected in the \ncytoplasm of the tegumental membrane and in the nuclei of the mesenchymal, testicular, ovarian and vitelline cells of 8-\nweek <jats:italic>Schistosoma japonicum<\/jats:italic>. In contrast, it was difficult to detect any signals of this sequence in 8-week <jats:italic>S. mansoni<\/jats:italic>, \nwhereas in 24-week male <jats:italic>S. mansoni<\/jats:italic> the signals were observed in the cytoplasm of the tegumental tubercles and in the \nnuclei of the mesenchymal and testicular cells. On the other hand, in 24-week female <jats:italic>S. mansoni<\/jats:italic> the signals were found \nin the nuclei of the mesenchymal, ovarian and vitelline cells but not found in the tegument. On the contrary, no \nhybridization band of the B2 sequence was detected in the amplified DNA of 3-week schistosomula of either species. \nThese observations proved that the host DNA sequences existed in restricted schistosome cells and were accumulated in \nthe schistosome body during their development.<\/jats:p>","DOI":"10.1017\/s0031182099004667","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"315-321","source":"Crossref","is-referenced-by-count":2,"title":["Localization of mouse type 2 <i>Alu<\/i> sequence in schistosomes"],"prefix":"10.1017","volume":"119","author":[{"given":"A.","family":"IMASE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"KUMAGAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"OHMAE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"IRIE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"IWAMURA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,9,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099004667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T19:01:37Z","timestamp":1554231697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099004667\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,9]]}},"alternative-id":["S0031182099004667"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099004667","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,9]]}}}