{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:48:48Z","timestamp":1761511728826},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1999,9,1]],"date-time":"1999-09-01T00:00:00Z","timestamp":936144000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:p>The tegumental membrane complex of <jats:italic>Schistosoma mansoni<\/jats:italic> is the site of interaction between the parasite and the host. \nThe tegument is involved in uptake of many nutrients, but also plays a crucial role in the evasion of the actions of the host \nimmune system. Essential for the success of this evasion is maintaining the integrity of the tegumental membranes. The \nrate of turnover of phospholipids was investigated by pulse-labelling worms cultured <jats:italic>in vitro<\/jats:italic>, followed by additional \nincubation in the presence of unlabelled substrates. Tegumental membranes were isolated, characterized using antibodies \nagainst specific tegumental proteins, and analysed. It was demonstrated that the most prominent fatty acid found in \ntegumental phospholipids, palmitate, incorporated rapidly into the phospholipid fraction during a 30 min pulse labelling. \nIn a subsequent 20 h chase with unlabelled substrates, the incorporated radioactivity was lost again from the tegumental \nmembrane complex. This high turnover of palmitate was found to be limited to phosphatidylcholine (PC) only. The \nturnover was due to deacylation\/reacylation, and not to the sloughing of membranes as is the case in schistosomula. It \nis speculated that this rapid turnover of PC in the tegument of adult schistosomes plays a new and important role in the \nimmune evasion by the parasite.<\/jats:p>","DOI":"10.1017\/s0031182099004679","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"287-294","source":"Crossref","is-referenced-by-count":28,"title":["Studies on phospholipid turnover argue against sloughing \nof tegumental membranes in adult <i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"119","author":[{"given":"J. F. H. M.","family":"BROUWERS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"SKELLY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M. G.","family":"VAN GOLDE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. G. M.","family":"TIELENS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,9,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099004679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T15:01:40Z","timestamp":1554217300000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099004679\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,9]]}},"alternative-id":["S0031182099004679"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099004679","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,9]]}}}