{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:09:31Z","timestamp":1762078171687},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2009,10,16]],"date-time":"2009-10-16T00:00:00Z","timestamp":1255651200000},"content-version":"unspecified","delay-in-days":3607,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,12]]},"abstract":"<jats:p>Detection of <jats:italic>Onchocerca volvulus<\/jats:italic> larvae in vector populations is of prime importance in the assessment of the effectiveness \nof onchocerciasis control programmes. Traditionally, detection of larvae is attained by the dissection of flies, but this time-consuming method cannot easily discriminate between species of <jats:italic>Onchocerca<\/jats:italic>. The genome of all <jats:italic>Onchocerca<\/jats:italic> species has \na unique 150 bp repeat, which can be amplified by PCR, and <jats:italic>O. volvulus<\/jats:italic>-specific DNA probes can detect these products \nby Southern blot (SB). This study optimizes a PCR\/SB assay, and compares it with fly dissection to estimate the prevalence \n(<jats:italic>p<\/jats:italic>) and intensity of infection (<jats:italic>m<\/jats:italic>) in the local vector population of a Mexican community that has become hypoendemic \nas a result of 7 years of treatment with ivermectin and nodulectomy. The PCR detected 1 infected fly in a pool of 99 \nuninfected flies, but the optimal pool size was 50 flies. At the community level, 1 out of 10550 flies was positive \n(<jats:italic>p<\/jats:italic>=0\u00b70095%, 95% confidence intervals CI=0\u00b700024\u201307\u00b705280%; <jats:italic>m<\/jats:italic>=0\u00b700027 larvae\/parous fly, CI=\u22120\u00b700026\u20130\u00b700081) \nby PCR, and 4 out of 10772 flies (<jats:italic>p<\/jats:italic>=0\u00b70371%, CI=0\u00b701012\u20130.09505%; <jats:italic>m<\/jats:italic>=0\u00b700107 larvae\/parous fly, 95% \nCI=0\u00b700002\u20130\u00b700212) by dissection (observed <jats:italic>m<\/jats:italic>=0\u00b70005). Both methods produce statistically similar estimates of the \nprevalence and intensity, indicating that pool screening is a viable alternative for entomological surveillance in areas where \nthe intensity of transmission is becoming extremely low as a result of control interventions.<\/jats:p>","DOI":"10.1017\/s0031182099005107","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"613-619","source":"Crossref","is-referenced-by-count":27,"title":["Detection of <i>Onchocerca volvulus<\/i> infection in <i>Simulium \nochraceum sensu lato<\/i>: comparison of a PCR assay and fly \ndissection in a Mexican hypoendemic community"],"prefix":"10.1017","volume":"119","author":[{"given":"M. A.","family":"RODR\u00cdGUEZ-P\u00c9REZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"DANIS-LOZANO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. H.","family":"RODR\u00cdGUEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. R.","family":"UNNASCH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"BRADLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,16]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T15:47:08Z","timestamp":1557416828000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005107\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,12]]}},"alternative-id":["S0031182099005107"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005107","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,12]]}}}