{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T13:44:02Z","timestamp":1778507042608,"version":"3.51.4"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>The ABA-1 protein of <jats:italic>Ascaris lumbricoides<\/jats:italic> (of humans) and <jats:italic>Ascaris suum<\/jats:italic> (of pigs) is abundant in the pseudocoelomic fluid \nof the parasites and also appears to be released by the tissue-parasitic larvae and the adult stages. The genes encoding the \npolyprotein precursor of ABA-1 (<jats:italic>aba-1<\/jats:italic>) were found to be arranged similarly in the two taxa, comprising tandemly \nrepeating units encoding a large polyprotein which is cleaved to yield polypeptides of approximately 15 kDa which fall \ninto 2 distinct classes, types A and B. The polyprotein possibly comprises only 10 units. The <jats:italic>aba-1<\/jats:italic> gene of <jats:italic>A. lumbricoides<\/jats:italic> \nis polymorphic, and the majority of substitutions observed occur in or near predicted loop regions in the encoded proteins. \nmRNA for ABA-1 is present in infective larvae within the egg, and in all parasitic stages, but was not detectable in \nunembryonated eggs. ABA-1 mRNA was confined to the gut of adult parasites, and not in body wall or reproductive \ntissues. Recombinant protein representing a single A-type unit for the <jats:italic>A. lumbricoides aba-1<\/jats:italic> gene was produced and found \nto bind retinol (Vitamin A) and a range of fatty acids, including the pharmacologically active lipids lysophosphatidic acid, \nlysoplatelet activating factor, and there was also evidence of binding to leukotrienes. It failed to bind to any of the \nanthelmintics screened. Differential Scanning Calorimetry showed that the recombinant protein was highly stable, and \nunfolded in a single transition at 90\u00b74 \u00b0C. Analysis of the transition indicated that the protein occurs as a dimer and that \nthe dimer dissociates simultaneously with the unfolding of the monomer units.<\/jats:p>","DOI":"10.1017\/s0031182099005363","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"211-224","source":"Crossref","is-referenced-by-count":56,"title":["The ABA-1 allergen of <i>Ascaris lumbricoides<\/i>: sequence \npolymorphism, stage and tissue-specific expression, lipid \nbinding function, and protein biophysical properties"],"prefix":"10.1017","volume":"120","author":[{"given":"Y.","family":"XIA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"SPENCE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"MOORE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"HEANEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"McDERMOTT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"COOPER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"WATSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"MEI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"KOMUNIECKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. W.","family":"KENNEDY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2000,2,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T19:23:21Z","timestamp":1554233001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005363\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["S0031182099005363"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005363","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}