{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:12:03Z","timestamp":1761707523038},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>Hookworms are gut-dwelling, blood-feeding nematodes that infect hundreds of millions of people, particularly in the \ntropics. As part of a program aiming to define novel drug targets and vaccine candidates for human parasitic nematodes, \ngenes expressed in adults of the human hookworm Necator americanus were surveyed by the expressed sequence tag \napproach. In total 161 new hookworm genes were identified. For the majority of these, a function could be assigned by \nhomology. The dataset includes proteases, protease inhibitors, a lipid binding protein, C-type lectins, an anti-bacterial \nfactor, globins and other genes of interest from a drug or vaccine development viewpoint. Three different classes of small, \nsecreted proteins were identified that may be involved in the host\u2013parasite interaction, including potential potassium \nchannel blocking peptides. One third of the genes were novel. These included highly expressed, secreted (glyco)proteins \nwhich may be part of the excretory\u2013secretory products of these important pathogens. Of particular interest are a family \nof 9 genes with similarity to the immunomodulatory protein, neutrophil inhibitory factor, that may play a role in \nestablishing an immunocompromised niche for this successful parasite.<\/jats:p>","DOI":"10.1017\/s0031182099005375","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"171-184","source":"Crossref","is-referenced-by-count":79,"title":["A survey of genes expressed in adults of the human \nhookworm, <i>Necator americanus<\/i>"],"prefix":"10.1017","volume":"120","author":[{"given":"J.","family":"DAUB","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"LOUKAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. I.","family":"PRITCHARD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"BLAXTER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2000,2,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T15:23:25Z","timestamp":1554218605000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005375\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["S0031182099005375"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005375","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}