{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:49:06Z","timestamp":1791118146706,"version":"4.1.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>Isolates of the protozoan parasite <jats:italic>Cryptosporidium parvum<\/jats:italic> have been differentiated into 2 genotypes: genotype \u2018H\u2019, which \nis associated only with human infections, and genotype \u2018C\u2019, which is associated with both human and animal infections. \nTo date, the analysis of polymorphisms of genes and of the small subunit ribosomal DNA have revealed no heterogeneity \nwithin the 2 genotypes. In the present study, a locus containing simple sequence repeats (microsatellites) was PCR \namplified and sequenced from 94 <jats:italic>C. parvum<\/jats:italic> isolates, which were collected from humans (immunocompetent and \nimmunocompromized individuals, outbreak and single cases) and from several animal hosts in 3 continents. The analysis \nrevealed that genotype \u2018H\u2019 can be further differentiated into 2 subgenotypes, and genotype \u2018C\u2019 can be further differentiated \ninto 4 subgenotypes. The 6 subgenotypes differ in terms of expansions\/contractions of the microsatellite repeats and by \npoint mutations. Some subgenotypes showed a wide geographical distribution, whereas others were restricted to specific \nregions. Therefore, microsatellites are informative markers for more defined studies on the epidemiology, the transmission \nroutes, and the population structure of this parasite.<\/jats:p>","DOI":"10.1017\/s0031182099005508","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"237-244","source":"Crossref","is-referenced-by-count":109,"title":["A microsatellite marker reveals population heterogeneity \nwithin human and animal genotypes of <i>Cryptosporidium \nparvum<\/i>"],"prefix":"10.1017","volume":"120","author":[{"given":"S.","family":"CACCI\u00d2","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"HOMAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"CAMILLI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"TRALDI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"KORTBEEK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"POZIO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2000,3,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T21:02:11Z","timestamp":1554498131000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005508\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["S0031182099005508"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005508","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}