{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:57:39Z","timestamp":1791262659404,"version":"4.1.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>Bidirectional signalling between <jats:italic>Trypanosoma brucei brucei<\/jats:italic> and CD8<jats:sup>+<\/jats:sup> \nT cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-\u03b3. Herein we further \ncharacterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed \nELISA. Secretion is induced by IFN-\u03b3 in a dose\u2013response manner, with 100 U\/ml giving maximal yields of bioactive \nTLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-\u03b3-induced \nsecretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower \nyields of TLTF in the culture supernatants.<\/jats:p>","DOI":"10.1017\/s0031182099005521","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:36:03Z","timestamp":1027776963000},"page":"281-287","source":"Crossref","is-referenced-by-count":14,"title":["Interferon-\u03b3 induces secretion of trypanosome lymphocyte \ntriggering factor via tyrosine protein kinases"],"prefix":"10.1017","volume":"120","author":[{"given":"M.","family":"HAMADIEN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"BAKHIET","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"HARRIS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2000,3,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005521","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T21:02:07Z","timestamp":1554498127000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005521\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["S0031182099005521"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005521","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}