{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:28:54Z","timestamp":1791404934514,"version":"4.3.3"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>This study was performed with <jats:italic>Litomosoides sigmodontis<\/jats:italic>, the only filarial species which can develop from the infective \nlarvae to the patent phase in immunocompetent laboratory BALB\/c mice. Parasitological features and immune responses \nwere analysed up to 3 months before and after challenge inoculation, by comparing 4 groups of mice: vaccinated \nchallenged, challenged only, vaccinated only, and naive mice. Male larvae were very susceptible to irradiation and only \nfemale irradiated larvae survived <jats:italic>in vivo<\/jats:italic>. Protection, assessed by a lower recovery rate, was confirmed and was established \nwithin the first 2 days of challenge. This early reduction of the recovery rate in vaccinated challenged mice was determined \nby their immune status prior to the challenge inoculation. This was characterized by high specific IgM and IgG subclass \n(IgG1, IgG2a and IgG3) levels, high specific IL-5 secretion from spleen cells <jats:italic>in vitro<\/jats:italic> and a high density of eosinophils \nin the subcutaneous connective tissue. Six h after the challenge inoculation, most tissue eosinophils were degranulated in \nvaccinated challenged mice. Thus, in the protocol of vaccination described, protection appeared mainly to result from the \nstimulation of a Th2 type response and eosinophils seemed to be the main effectors for the increased killing of infective \nlarvae in vaccinated challenged mice. Two months after challenge inoculation, the percentage of microfilaraemic mice was \nlower in vaccinated challenged mice as a consequence of this overall reduction in the worm load. In both vaccinated \nchallenged and challenged only groups, the <jats:italic>in vitro<\/jats:italic> splenocyte proliferative capacity was reduced in microfilaraemic mice.<\/jats:p>","DOI":"10.1017\/s0031182099005533","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"271-280","source":"Crossref","is-referenced-by-count":45,"title":["Parasitology and immunology of mice vaccinated with \nirradiated <i>Litomosoides sigmodontis<\/i> larvae"],"prefix":"10.1017","volume":"120","author":[{"given":"L.","family":"LE GOFF","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"MARTIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. P.","family":"OSWALD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. N.","family":"VUONG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"PETIT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. N.","family":"UNGEHEUER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"BAIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2000,3,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005533","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T21:02:13Z","timestamp":1554498133000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005533\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["S0031182099005533"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005533","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}