{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T01:18:07Z","timestamp":1750468687555},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>A novel acidic extracellular single-stranded endonuclease was demonstrated for the first time in the excretory\u2013secretory \n(E\u2013S) products of 2 species of <jats:italic>Trichinella<\/jats:italic>. Unlike the double-stranded endonuclease reported earlier, the single-stranded \nmolecule is divalent cation independent and is detected in both <jats:italic>T. spiralis<\/jats:italic> and <jats:italic>T. pseudospiralis<\/jats:italic> E\u2013S products. It hydrolysed \nsingle-stranded DNA and RNA at comparable rates. The single-stranded endonuclease was sensitive to inhibition by \nZn<jats:sup>2+<\/jats:sup> and to high concentrations of NaCl. Zymographic analysis indicated that it was encoded by at least 3 peptides of \n<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> \u223c 50\u201360 kDa. The rate of hydrolysis of single-stranded targets by the E\u2013S products was substantially higher than that \nof the double-stranded molecule. Due to the differences in peptide profile, divalent cation dependence, and species-specific \nexpression, the single and double-stranded endonucleases are likely to be encoded by different proteins and may have \ndifferent functions.<\/jats:p>","DOI":"10.1017\/s0031182099005879","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"527-533","source":"Crossref","is-referenced-by-count":11,"title":["Single-stranded endonuclease activity in the \nexcretory\u2013secretory products of <i>Trichinella spiralis<\/i> and \n<i>Trichinella pseudospiralis<\/i>"],"prefix":"10.1017","volume":"120","author":[{"given":"C. H.","family":"MAK","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Y. Y.","family":"CHUNG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. C.","family":"KO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2000,5,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099005879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T23:26:26Z","timestamp":1554506786000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099005879\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["S0031182099005879"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099005879","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}