{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:42:17Z","timestamp":1791182537426,"version":"4.1.0"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2015,1,2]],"date-time":"2015-01-02T00:00:00Z","timestamp":1420156800000},"content-version":"unspecified","delay-in-days":10868,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infect. Control"],"published-print":{"date-parts":[[1985,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the mode of transmission of<jats:italic>L. pneumophila<\/jats:italic>is as yet unclear, the hot water distribution system has been shown to be the reservoir for Legionella within the hospital environment. In this report we identify a previously unrecognized reservoir for<jats:italic>L. pneumophila<\/jats:italic>within the hospital environment, ie, the cold water dispensers of hospital ice machines. The cold water dispensers of 14 ice machines were cultured monthly over a 1-year period. Positive cultures were obtained from 8 of 14 dispensers, yielding from 1 to 300 CFU\/plate. We were able to link the positivity of these cold water sites to the incoming cold water supply by recovering<jats:italic>L. pneumophila<\/jats:italic>from the cold water storage tank, which is directly supplied by the incoming municipal water line. This was accomplished by a novel enrichment experiment designed to duplicate the conditions (temperature, sediment, stagnation, and continuous seeding) of the hot water system. Our data indicate that significant contamination of cold water outlets with<jats:italic>L. pneumophila<\/jats:italic>can occur. Although no epidemiologic link to disease was made, the fact that the primary source of a patient's drinking water is from the ice machines warrants further investigation of these water sources as possible reservoirs.<\/jats:p>","DOI":"10.1017\/s0195941700062937","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T04:54:44Z","timestamp":1470200084000},"page":"141-146","source":"Crossref","is-referenced-by-count":41,"title":["Isolation of<i>Legionella pneumophila<\/i>from the Cold Water of Hospital Ice Machines: Implications for Origin and Transmission of the Organism"],"prefix":"10.1017","volume":"6","author":[{"given":"Janet E.","family":"Stout","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor L.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Muraca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2015,1,2]]},"reference":[{"key":"S0195941700062937_ref007","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1128\/aem.46.3.769-770.1983","article-title":"Relationship between colonization of hospital buildings with Legionella pneumophila and hot water temperatures","volume":"46","author":"Plouffe","year":"1983","journal-title":"Appl Environ Microbiol"},{"key":"S0195941700062937_ref013","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/jcm.14.3.298-303.1981","article-title":"Improved semiselective medium for the isolation of Legionella pneumophila from contaminated clinical and environmental specimens","volume":"14","author":"Edelstein","year":"1981","journal-title":"J Clin Microbiol"},{"key":"S0195941700062937_ref006","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198202253060807"},{"key":"S0195941700062937_ref003","doi-asserted-by":"crossref","unstructured":"Johnson J , Yu VL , Best M , et al: Nosocomial Legionella pneumonia in a population of head and neck cancer patients. Laryngoscope 1985, to be published.","DOI":"10.1288\/00005537-198512000-00006"},{"key":"S0195941700062937_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)90290-8"},{"key":"S0195941700062937_ref014","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1128\/aem.49.1.221-228.1985","article-title":"The ecology of L. pneumophila in water distribution systems","volume":"49","author":"Stout","year":"1985","journal-title":"Appl Environ Microbiol"},{"key":"S0195941700062937_ref011","volume-title":"Aspiration of contaminated water may be a mode of transmission for Legionella pneumophila","author":"Yu","year":"1981"},{"key":"S0195941700062937_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)91944-4"},{"key":"S0195941700062937_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)91449-0"},{"key":"S0195941700062937_ref009","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198002143020703"},{"key":"S0195941700062937_ref001","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1983.03330470024025"},{"key":"S0195941700062937_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0196-6553(84)90069-5"},{"key":"S0195941700062937_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(82)90727-6"},{"key":"S0195941700062937_ref010","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/146.4.460"},{"key":"S0195941700062937_ref005","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1128\/aem.43.5.1104-1110.1982","article-title":"Hot water systems as sources of Legionella pneumophila in hospital and non-hospital plumbing fixtures","volume":"43","author":"Wadowsky","year":"1982","journal-title":"Appl Environ Microbiol"}],"container-title":["Infection Control"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0195941700062937","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T20:24:05Z","timestamp":1656966245000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0195941700062937\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,4]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1985,4]]}},"alternative-id":["S0195941700062937"],"URL":"https:\/\/doi.org\/10.1017\/s0195941700062937","relation":{},"ISSN":["0195-9417","2327-9451"],"issn-type":[{"value":"0195-9417","type":"print"},{"value":"2327-9451","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,4]]}}}