{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:27:08Z","timestamp":1781108828581,"version":"3.54.1"},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"S4","license":[{"start":{"date-parts":[[2015,9,18]],"date-time":"2015-09-18T00:00:00Z","timestamp":1442534400000},"content-version":"unspecified","delay-in-days":10548,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Neurol. Sci."],"published-print":{"date-parts":[[1986,11]]},"abstract":"<jats:title>Abstract:<\/jats:title><jats:p>Postmortem human brain samples were taken from non-neurological controls as well as demented subjects who died with Alzheimer's disease (AD), multi-infarct dementia (MID), or a combination of AD and MID dementia (MIXED). Choline acetyltransferase (Ch AT) activity was measured radiometrically using [ 1 -<jats:sup>l4<\/jats:sup>C]acetyl-coenzyme A as the substrate, muscarinic binding was assayed with [<jats:sup>3<\/jats:sup>H]quinuclidinyl benzilate, and the proportion of binding associated with high affinity agonist sites was measured by carbamylcholine displacement of the radioligand.<\/jats:p><jats:p>Relative to control, ChAT activity was significantly reduced (p \u22640.01) in samples taken from the temporal, frontal, and hippocampal areas of demented patients. A small elevation in Bmax was noted in the hippocampal endplate (p \u22640.01) (AD vs. control) and the H<jats:sub>1<\/jats:sub>-subiculum region (p \u22640.05) (AD vs. all other groups). In addition, the percentage of binding associated with high affinity agonist sites was greater in the frontal cortex of AD and MID samples (p\u22640.05), compared with MIXED and control, and in the temporal cortex of the AD group compared with all other groups (p\u22640.05). The results suggest a regionally specific upregulation of cerebral muscarinic receptors in dementia, especially in AD.<\/jats:p>","DOI":"10.1017\/s0317167100037252","type":"journal-article","created":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T11:38:05Z","timestamp":1469705885000},"page":"528-532","source":"Crossref","is-referenced-by-count":45,"title":["Muscarinic Binding and Choline Acetyltransferase in Postmortem Brains of Demented Patients"],"prefix":"10.1017","volume":"13","author":[{"given":"Steven B.","family":"Waller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melvyn J.","family":"Ball","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Reynolds","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edythe 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