{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:06:29Z","timestamp":1768824389805,"version":"3.49.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"unspecified","delay-in-days":90,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>Two cases of <jats:italic>Escherichia coli<\/jats:italic> O157 infection occurred in children after visiting an inner city \nopen farm. Subsequently faecal samples collected from animal pens and samples of composted \nmixed animal manure and vegetable waste were examined for <jats:italic>E. coli<\/jats:italic> O157 by enrichment \nculture, immunomagnetic separation and culture of magnetic beads to cefixime tellurite sorbitol \nMacConkey agar. Strains of <jats:italic>E. coli<\/jats:italic> O157 were characterized by hybridization with DNA \nprobes for VT1, VT2 and <jats:italic>eaeA<\/jats:italic>, plasmid profile analysis, phage typing and pulsed field gel \nelectrophoresis (PFGE). Verocytotoxin-producing <jats:italic>E. coli<\/jats:italic> O157 strains were isolated from faecal \nsamples from a cow, a horse, 3 breeds of pigs, 2 breeds of sheep and 2 breeds of goats and \nfrom 2 samples of compost which had been processed for 3 months. All strains were phage \ntype 21, hybridized with probes for VT2 and <jats:italic>eaeA<\/jats:italic> but not with one for VT1, harboured 92 \nand 2 kb plasmids and gave indistinguishable banding patterns with PFGE. Although only two \nculture-confirmed cases of infection had been identified, the farm had over 100 000 visitors per \nyear and so it was closed as a precaution both to allow a thorough investigation and to \nprevent further cases. The investigation identified many factors which may have contributed to \ntransmission of <jats:italic>E. coli<\/jats:italic> O157 infection. Most of these were readily resolved by appropriate \ncorrective measures and as there were no further cases associated with the farm during the \nensuing 4 weeks it then re-opened. These cases highlight the risk, especially to young children, \nof acquiring zoonotic infections during visits to open farms and emphasize the need for \nadequate guidance and supervision before and during such visits.<\/jats:p>","DOI":"10.1017\/s0950268800004775","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:29:39Z","timestamp":1027776579000},"page":"531-536","source":"Crossref","is-referenced-by-count":50,"title":["Infection with verocytotoxin-producing <i>Escherichia coli<\/i> O157 during a visit to an inner city open farm"],"prefix":"10.1017","volume":"125","author":[{"given":"P. A.","family":"CHAPMAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"CORNELL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"GREEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2001,3,1]]},"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800004775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T19:48:40Z","timestamp":1557344920000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800004775\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["S0950268800004775"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800004775","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]}}}