{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T06:48:25Z","timestamp":1648882105271},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":6375,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1991,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Characterization of 169 strains<jats:italic>of Salmonella typhi<\/jats:italic>of phage types C<jats:sub>1<\/jats:sub>, C<jats:sub>4<\/jats:sub>, D<jats:sub>1<\/jats:sub>and D<jats:sub>9<\/jats:sub>isolated in 1975\u201388 was carried out by rDNA gene restriction pattern analysis. Twenty-four isolates had been recovered during four large waterbone outbreaks in the last 20 years in Sicily; 145 strains, isolated from apparently sporadic cases of infection in Southern Italy in the same period of time, were also examined.<\/jats:p><jats:p>Application of rRNA\u2013DNA hybridization technique after digestion of chromosomal DNA with<jats:italic>Cla<\/jats:italic>I showed the identity of patterns of the epidemic strains of phage types C<jats:sub>1<\/jats:sub>and D<jats:sub>1<\/jats:sub>, confirming attribution of the outbreaks to single bacterial clones. Patterns of the two available strains of lysotype D<jats:sub>9<\/jats:sub>were slightly different, whilst the 12 epidemic strains of phage type C<jats:sub>4<\/jats:sub>could be assigned to two distinct patterns scarcely related to each other and, consequently, to two different clones. A considerable heterogeneity was detected among all apparently sporadic isolates of the four phage types under study.<\/jats:p><jats:p>This fingerprinting method appears a reliable tool to complement phage typing in characterizing isolates of<jats:italic>S. typhi.<\/jats:italic>In particular, epidemiological features of spread of this salmonella serovar in areas, where simultaneous circulation of indigenous and imported strains occurs, can be elucidated.<\/jats:p>","DOI":"10.1017\/s0950268800049268","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T09:09:37Z","timestamp":1242378577000},"page":"565-576","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":25,"title":["rDNA fingerprinting as a tool in epidemiological analysis of<i>Salmonella typhi<\/i>infections"],"prefix":"10.1017","volume":"107","author":[{"given":"A.","family":"Nastasl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Mammina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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