{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:21:59Z","timestamp":1780586519957,"version":"3.54.1"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":6253,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>An indirect ELISA developed for the serological detection of <jats:italic>Salmonella typhimurium<\/jats:italic> in chickens using lipopolysaccharide as detecting antigen has been evaluated further in experimental infections. Following oral infection of 24-week-old laying hens with an invasive strain of <jats:italic>S. typhimurium<\/jats:italic>, high titres of specific circulating IgG were induced which were maintained for 20 weeks. Similar IgG titres were found in egg yolk. When 4-day-old chickens were infected high antibody titres persisted for 45 weeks. Chickens inoculated orally or intramuscularly with different numbers of <jats:italic>S. typhimurium<\/jats:italic> organisms showed graded serum IgG responses to LPS. The IgG titres in experimentally infected in-bred lines of chickens which showed greater genetic resistance to salmonella infection were significantly lower than those found in more susceptible lines. Oral and intramuscular infection with 18 different types of enterobacteria, including avian pathogenic <jats:italic>E. coli, Citrobacter<\/jats:italic> spp., <jats:italic>Klebsiella<\/jats:italic> spp., <jats:italic>Proteus<\/jats:italic> spp. and citrobacter-like organisms possessing some salmonella LPS (none possessed the 0\u20134 antigen) and flagella antigens, did not induce <jats:italic>S. typhimurium<\/jats:italic> LPS-specific IgG responses. Chickens infected orally with rough or non-flagellate mutants of <jats:italic>S. typhimurium<\/jats:italic> did not induce high titres of LPS or flagella-specific IgG respectively. Sera obtained from <jats:italic>S. typhimurium<\/jats:italic>-infected chickens showed much higher titres against <jats:italic>S. typhimurium<\/jats:italic> LPS than with those antigens from other serotypes, including <jats:italic>S. enteritidis<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0950268800049712","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T05:08:04Z","timestamp":1242364084000},"page":"231-242","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":24,"title":["Further observations on the serological response to experimental <i>Salmonella typhimurium<\/i> in chickens measured by ELISA"],"prefix":"10.1017","volume":"108","author":[{"given":"P. A.","family":"Barrow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,5,15]]},"reference":[{"key":"S0950268800049712_ref009","first-page":"339","article-title":"Experimental infection of egg-laying hens with Salmonella enteritidis phage type 4","volume":"20","author":"Barrow","year":"1991","journal-title":"Avian Pathol"},{"key":"S0950268800049712_ref011","doi-asserted-by":"publisher","DOI":"10.2307\/1591614"},{"key":"S0950268800049712_ref015","first-page":"501","article-title":"Serological analysis of chicken flocks for antibodies to Salmonella enteritidis","volume":"127","author":"Chart","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref010","doi-asserted-by":"publisher","DOI":"10.1136\/vr.128.4.74"},{"key":"S0950268800049712_ref017","doi-asserted-by":"publisher","DOI":"10.1136\/vr.128.2.43"},{"key":"S0950268800049712_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800054534"},{"key":"S0950268800049712_ref024","article-title":"The serological response of chickens to infection with Salmonella gallinarum-pullorum detected by ELISA","author":"Barrow","year":"1991","journal-title":"Avian Dis"},{"key":"S0950268800049712_ref019","doi-asserted-by":"publisher","DOI":"10.1080\/00071668808417078"},{"key":"S0950268800049712_ref005","first-page":"519","article-title":"Antibody response to experimental Salmonella typhimurium infection in chickens measured by ELISA","volume":"126","author":"Hassan","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref006","first-page":"567","article-title":"Serological and bacteriological investigation of chickens from flocks naturally infected with Salmonella enteritidis","volume":"125","author":"Cooper","year":"1989","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref025","doi-asserted-by":"publisher","DOI":"10.1136\/vr.125.21.531"},{"key":"S0950268800049712_ref012","first-page":"147","article-title":"Detection of Salmonella","volume":"126","author":"Nicholas","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref003","first-page":"586","article-title":"Detection of Salmonella infection by ELISA","volume":"125","author":"Barrow","year":"1989","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref004","first-page":"219","article-title":"Egg yolk antibody detection in identification of Salmonela infected poultry","volume":"126","author":"Dadrast","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref016","doi-asserted-by":"publisher","DOI":"10.1136\/vr.128.9.215-a"},{"key":"S0950268800049712_ref020","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400047306"},{"key":"S0950268800049712_ref014","first-page":"92","article-title":"Serological tests for Salmonella enteritidis in chickens","volume":"126","author":"Chart","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref002","unstructured":"2. Nagaraja KV , Ausherman LT , Emery DA , Pomeroy BS . Update on enzyme linked immunosorbent assay for its field application in the detection of Salmonella arizonae infection in breeder flocks of turkeys. Proceedings of the 29th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians 1986; 347\u201356."},{"key":"S0950268800049712_ref021","doi-asserted-by":"publisher","DOI":"10.1080\/03079458808436478"},{"key":"S0950268800049712_ref008","first-page":"20","article-title":"Serological tests for Salmonella enteritidis in chickens","volume":"126","author":"Minga","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref013","first-page":"20","article-title":"Serological tests for Salmonella enteritidis in chickens","volume":"129","author":"Chart","year":"1990","journal-title":"Vet Rec"},{"key":"S0950268800049712_ref022","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-101-1-121"},{"key":"S0950268800049712_ref001","unstructured":"1. Nagaraja KV , Emery DA , Sherlock LF , Newman JS , Pomeroy BS . Detection of Salmonella arizonae in turkey flocks by ELISA. Proceedings of the 27th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians 1984: 185\u2013204."},{"key":"S0950268800049712_ref023","volume-title":"Molecular cloning \u2013 a laboratory manual","author":"Sambrook","year":"1989"},{"key":"S0950268800049712_ref026","doi-asserted-by":"publisher","DOI":"10.1080\/03079459108418749"},{"key":"S0950268800049712_ref018","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/S0034-5288(18)30685-4","article-title":"Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens","volume":"42","author":"Barrow","year":"1987","journal-title":"Res Vet Sci"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800049712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T18:04:06Z","timestamp":1558029846000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800049712\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["S0950268800049712"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800049712","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]},"assertion":[{"value":"Cambridge University Press 1992","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}