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One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to<jats:italic>Rickettsia conorii<\/jats:italic>, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.<\/jats:p>","DOI":"10.1017\/s0950268800050056","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T09:06:21Z","timestamp":1242378381000},"page":"555-557","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain"],"prefix":"10.1017","volume":"108","author":[{"given":"C.","family":"Herrero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Pelaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Mart\u00edn-Bourgon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,5,15]]},"reference":[{"key":"S0950268800050056_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1981.30.230"},{"key":"S0950268800050056_ref006","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1128\/IAI.37.2.779-785.1982","article-title":"Reactivation of Rickettsia rickettsii in Dermacentor andersoni ticks: an ultrastructural analysis","volume":"37","author":"Hayes","year":"1982","journal-title":"Infect Immun"},{"key":"S0950268800050056_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)90369-5"},{"key":"S0950268800050056_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)90037-X"},{"key":"S0950268800050056_ref001","first-page":"1","article-title":"Fiebre botonosa en Espa\u00f1a 1983\u20131987","volume":"8","journal-title":"Bol Microbiol Sem 1988"},{"key":"S0950268800050056_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00146379"},{"key":"S0950268800050056_ref008","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1128\/JCM.25.1.167-171.1987","article-title":"Reactivity of monoclonal antibodies to Rickettsia rickettsii with Spotted Fever and Typhus group rickettsiae","volume":"25","author":"Anacker","year":"1987","journal-title":"J Clin Microbiol"},{"key":"S0950268800050056_ref007","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.2460\/ajvr.1985.46.10.2124","article-title":"Canine Rocky Mountain spotted fever: a kennel epizootic","volume":"46","author":"Breitschwerdt","year":"1985","journal-title":"Am J Vet Res"},{"key":"S0950268800050056_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.6.1158"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800050056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T12:27:53Z","timestamp":1739104073000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800050056\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":9,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,6]]}},"alternative-id":["S0950268800050056"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800050056","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"type":"print","value":"0950-2688"},{"type":"electronic","value":"1469-4409"}],"subject":[],"published":{"date-parts":[[1992,6]]},"assertion":[{"value":"Cambridge University Press 1992","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}