{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T18:56:49Z","timestamp":1761418609702},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":5947,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Haemolytic uraemic syndrome (HUS) is a leading cause of acute renal failure in childhood. Although infection with<jats:italic>Escherichia coli<\/jats:italic>O 157. H7 has been associated with HUS in North America and Europe, only a limited number of studies have examined the role of other verotoxin-producing<jats:italic>E. coli<\/jats:italic>(VTEC) serotypes in this condition. To address this issue, we conducted a comprehensive, prospective microbiological study of patients treated for HUS at eight Canadian hospitals in the summer of 1990. Of the 34 consecutive patients with HUS enrolled over 4 months,<jats:italic>E. coli<\/jats:italic>0 157. H7 was isolated from the stools of 26, and other<jats:italic>E. coli<\/jats:italic>serotypes were isolated from four patients. In four subjects no pathogenic<jats:italic>E. coli<\/jats:italic>serotypes were identified on stool culture. Using oligonucleotide probes specific for VT-1 and VT-2, verotoxin genes were detected in the stool isolates of all patients with<jats:italic>E. coli<\/jats:italic>O 157.H7, and from two with other<jats:italic>E. coli<\/jats:italic>serotypes. Two other patients had at least a fourfold rise in anti-verotoxin antibodies. Strong evidence of exposure to a verotoxin was present in 30\/34 (88%). Patients with<jats:italic>E. coli<\/jats:italic>0 157.H7 infection were more likely to develop an antibody response to VT-2 than to VT-1 (22\/22 vs 12\/22; P = 0.002). These results further strengthen the association of HUS with verotoxin-producing<jats:italic>E. coli<\/jats:italic>in North America, and confirm that<jats:italic>E. coli<\/jats:italic>serotypes other than 0 157. H7 are isolated in a small proportion of summertime HUS episodes.<\/jats:p>","DOI":"10.1017\/s0950268800050615","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T09:02:28Z","timestamp":1242378148000},"page":"1-7","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":45,"title":["A prospective study of exposure to verotoxin-producing<i>Escherichia coli<\/i>among Canadian children with haemolytic uraemic syndrome"],"prefix":"10.1017","volume":"110","author":[{"given":"P. C.","family":"Rowe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Orrbine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Lior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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