{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:46:07Z","timestamp":1761662767107},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":5401,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>An enzyme immunoassay (EIA) for astrovirus type 1 together with immune electronmicroscopy (IBM) was used to type a collection of 162 astroviruses obtained from 1981\u201393 from children with diarrhoea. The EIA was found to be specific for astrovirus type 1. Astrovirus types 2\u20134 were typed by IEM.<\/jats:p><jats:p>Astrovirus type 1 was the prevalent serotype 107\/125 (86%), followed by type 3 (8%), type 4 (6%) and type 2 (1 %). Six samples containing astrovirus could not be typed or detected by EIA because they were coated with coproantibodies; 11 others were not identified. Virus particles could no longer be detected in 15\/162 (9%) samples following storage for<jats:italic>\u2265<\/jats:italic>2 years.<\/jats:p><jats:p>Selected samples containing astrovirus types 1\u20134 were passaged in CaCO<jats:sub>2<\/jats:sub>cells and their identity confirmed by one or both assays. One sample was shown to have remained viable for 10 years when stored as an aqueous suspension at \u221220 \u00b0C.<\/jats:p><jats:p>Two patients with severe combined immune deficiency disease (SCID) were shown to be excreting astrovirus type 1 for 32 and 102 days respectively. One child was simultaneously shedding rotavirus and the other child was excreting adenovirus.<\/jats:p>","DOI":"10.1017\/s0950268800051578","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T08:48:10Z","timestamp":1242377290000},"page":"153-159","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":52,"title":["Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981\u201393"],"prefix":"10.1017","volume":"113","author":[{"given":"J.","family":"Noel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Cubitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,5,15]]},"reference":[{"key":"S0950268800051578_ref014","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.32.8.786"},{"key":"S0950268800051578_ref004","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890030308"},{"key":"S0950268800051578_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)92101-9"},{"key":"S0950268800051578_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(92)90140-9"},{"key":"S0950268800051578_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)92581-7"},{"key":"S0950268800051578_ref011","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.2.226"},{"key":"S0950268800051578_ref012","first-page":"324","article-title":"Astrovirus as a cause of gastroenteritis in children","author":"Herrmann","year":"1991","journal-title":"N Eng J Med"},{"key":"S0950268800051578_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90158-9"},{"key":"S0950268800051578_ref008","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800057551"},{"key":"S0950268800051578_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)90020-3"},{"key":"S0950268800051578_ref006","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-57-2-421"},{"key":"S0950268800051578_ref010","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.1.182"},{"key":"S0950268800051578_ref019","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890240410"},{"key":"S0950268800051578_ref009","first-page":"651","volume-title":"Virus infections of the gut and liver","volume":"4","author":"Cubitt","year":"1990"},{"key":"S0950268800051578_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF01318354"},{"key":"S0950268800051578_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(77)90338-5"},{"key":"S0950268800051578_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)90858-2"},{"key":"S0950268800051578_ref013","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1128\/JCM.30.5.1140-1144.1992","article-title":"Astrovirus associated diarrhoea among Guatemalan ambulatory rural children","volume":"30","author":"Cruz","year":"1992","journal-title":"J Clin Microbiol"},{"key":"S0950268800051578_ref018","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800049049"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800051578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T05:04:21Z","timestamp":1589778261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800051578\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,8]]}},"alternative-id":["S0950268800051578"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800051578","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8]]},"assertion":[{"value":"Cambridge University Press 1994","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}