{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:27:37Z","timestamp":1753921657394},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":4792,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1996,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Ribotyping performed with six restriction endonucleases was used to study the molecular epidemiology of shigellosis in Asturias, Spain. The series included<jats:italic>Shigella sonnei<\/jats:italic>from 34 sporadic cases, 3 outbreaks and 3 reference strains, and<jats:italic>S. flexneri<\/jats:italic>from 13 sporadic cases and 1 reference strain. The<jats:italic>S. sonnei<\/jats:italic>strains were grouped into 5 ribotypes with<jats:italic>Sal<\/jats:italic>I, 4 with<jats:italic>Hind<\/jats:italic>III and<jats:italic>Pvu<\/jats:italic>II, 3 with<jats:italic>Bgl<\/jats:italic>II and<jats:italic>Eco<\/jats:italic>R I and 2 with<jats:italic>Hinc<\/jats:italic>II (Discriminatory Index (DI) between 0\u00b754 and 0\u00b714); the<jats:italic>S. flexneri<\/jats:italic>into 5 ribotypes with<jats:italic>Sal<\/jats:italic>I, Hinc II and<jats:italic>Hind<\/jats:italic>III, and 4 with the other enzymes (DI = 0\u00b771\u20130\u00b763). The combination of results for 2 or more enzymes facilitated an additional discrimination, the highest values in<jats:italic>S. sonnei<\/jats:italic>were for the 6 enzymes (16 types, DI = 0\u00b791) and in<jats:italic>S. flexneri<\/jats:italic>for some combinations of 3 or more enzymes (7 types, DI = 0\u00b781). Ribotypes with the 6 enzymes defined 16 clonal lines in<jats:italic>S. sonnei<\/jats:italic>and 7 in<jats:italic>S. flexneri<\/jats:italic>, which showed a different degree of genetic heterogeneity, and all the lines of each species falling into a different cluster. No line appeared as clearly endemic in the bowels of Asturian people.<\/jats:p>","DOI":"10.1017\/s0950268800052353","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T08:34:17Z","timestamp":1242376457000},"page":"127-135","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Usefulness of ribotyping in a molecular epidemiology study of shigellosis"],"prefix":"10.1017","volume":"116","author":[{"given":"M. C.","family":"Mendoza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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