{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T20:11:47Z","timestamp":1694635907807},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"unspecified","delay-in-days":4914,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The prevalence of antibodies against<jats:italic>Entamoeba histolytica<\/jats:italic>was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of<jats:italic>Entamoeba histolytica<\/jats:italic>HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied<jats:italic>by<\/jats:italic>the National Seroepidemiology Survey, antibodies were found in 4\u00b749 % (4\u00b732\u2013;4\u00b765% at 95 % confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1\u00b725:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country.<\/jats:p><jats:p>Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame.<\/jats:p>","DOI":"10.1017\/s0950268800058702","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T08:38:04Z","timestamp":1242376684000},"page":"535-543","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence of antibodies against<i>Entamoeba histolytica<\/i>in Mexico measured by ELISA"],"prefix":"10.1017","volume":"115","author":[{"given":"C. R.","family":"Gonz\u00e1lez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Isibasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Ortiz-Navarrete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Paniagua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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