{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:15:21Z","timestamp":1762323321305},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":7993,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1987,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Investigations using a liquid-phase blocking sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies against foot-and-mouth disease virus (FMDV) in sera from sheep and from cattle are reported, and results compared with those obtained by virus neutralization (VN) tests.<\/jats:p><jats:p>Serum antibody titres in sheep after primary vaccination and in cattle challenged with a natural aerosol after vaccination were similar by ELISA and VN. However, the antibody levels detected in sera of cattle during early infection and of vaccinated cattle after intradermolingual challenge were clearly greater by ELISA than by VN.<\/jats:p><jats:p>The ELISA titres in cattle sera following synthetic peptide vaccination indicated some relationship to protection and were clearly different from those recorded by VN. On the other hand, the antibody levels following conventional vaccination showed that ELISA and VN titres in cattle sera were related to protection. Although there was a good agreement between the ELISA antibody titre and protection for the four vaccines used, by VN the titre which afforded protection varied depending on the vaccine used.<\/jats:p><jats:p>The ELISA was considered therefore to be more reliable than the VN and may prove useful for evaluating the immunological response of animals following infection and following vaccination.<\/jats:p>","DOI":"10.1017\/s0950268800066590","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T12:44:02Z","timestamp":1255956242000},"page":"733-744","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":57,"title":["Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus: III. Evaluation of antibodies after infection and vaccination"],"prefix":"10.1017","volume":"99","author":[{"given":"C.","family":"Hamblin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. P.","family":"Kitching","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. I.","family":"Donaldson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Crowther","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. T. R.","family":"Barnett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"S0950268800066590_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90441-2"},{"key":"S0950268800066590_ref006","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0034-5288(18)33445-3","article-title":"Radial immunodiffusion and serum neutralisation techniques for the assay of antibodies to swine vesicular disease","volume":"20","author":"Golding","year":"1976","journal-title":"Research in Veterinary Science"},{"key":"S0950268800066590_ref014","first-page":"57","article-title":"The relationship of neutralizing antibody titres for foot-and-mouth disease virus and the protection of cattle","volume":"39\u201340","author":"Sutmoller","year":"1980","journal-title":"Boletin del Centro Panamericano de Fiebre Aflosa"},{"key":"S0950268800066590_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-1157(84)80062-1"},{"key":"S0950268800066590_ref005","doi-asserted-by":"publisher","DOI":"10.20506\/rst.3.3.173"},{"key":"S0950268800066590_ref003","doi-asserted-by":"publisher","DOI":"10.1126\/science.3008333"},{"key":"S0950268800066590_ref013","first-page":"52","volume-title":"Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Lelystad, Netherlands, sept. 1983","author":"Pay","year":"1983"},{"key":"S0950268800066590_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/BF01318244"},{"key":"S0950268800066590_ref004","doi-asserted-by":"crossref","unstructured":"Donaldson A. I. , Gibson C. F. , Oliver R. , Hamblin C. & Kitching R. P. (1987). Infection of cattle by airborne foot-and-mouth disease virus: minimal doses with O1 and SAT 2 strains. Research and Veterinary Science. In press.","DOI":"10.1016\/S0034-5288(18)30804-X"},{"key":"S0950268800066590_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90442-4"},{"key":"S0950268800066590_ref009","first-page":"44","volume-title":"Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Lelystad, Netherlands, Sept. 1983","author":"Have","year":"1983"},{"key":"S0950268800066590_ref010","doi-asserted-by":"publisher","DOI":"10.1007\/BF01863914"},{"key":"S0950268800066590_ref011","first-page":"252\u2013261","article-title":"Relation entre titre d'anticorps neutralisants et protection des animaux apr\u00e8s vaccination antiaphteuse","volume":"103","author":"Mackowiak","year":"1962","journal-title":"Annales de l'Institut Pasteur"},{"key":"S0950268800066590_ref012","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0034-5288(18)34978-6","article-title":"The tissue culture colour test for assaying the virus and neutralizing antibody of foot-and-mouth disease and its application to the measurement of immunity in cattle","volume":"2","author":"Martin","year":"1961","journal-title":"Research in Veterinary Science"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800066590","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T21:18:02Z","timestamp":1558387082000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800066590\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,12]]}},"alternative-id":["S0950268800066590"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800066590","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,12]]},"assertion":[{"value":"Cambridge University Press 1987","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}