{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T18:42:44Z","timestamp":1726425764686},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":6045,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Haemophilus ducreyi<\/jats:italic>, the causal organism of chancroid, has increased in significance recently due to its association with HIV transmission. Most previous typing systems have exploited phenotypie characteristics. Detection of ribosomal RNA cistrons, ribotyping, was successfully developed to examine H.<jats:italic>ducreyi<\/jats:italic>, but required the use of<jats:sup>32<\/jats:sup>P.<\/jats:p><jats:p>We have used digoxigenin to define ribotypes from 30 strains of<jats:italic>H. ducreyi<\/jats:italic>from diverse geographical locations. This was achieved by agarose gel electrophoresis of restriction enzyme (RE) digested DNA extracts. These extracts were vacublotted onto nylon membrane and probed using digoxigenin labelled complementary DNA probe, prepared from<jats:italic>Escherichia coli<\/jats:italic>16S and 23S ribosomal RNA. From 19 REs tested,<jats:italic>Ava<\/jats:italic>II,<jats:italic>Hinc<\/jats:italic>II,<jats:italic>Bgl<\/jats:italic>II and<jats:italic>Bst<\/jats:italic>E II gave clear ribotypes. The ribotypes of<jats:italic>Bst<\/jats:italic>E II and<jats:italic>Bgl<\/jats:italic>II used together gave the highest index of discrimination (D = 0\u00b795), 16 types, and showed good reproducibility. This nonradioactive method demonstrates the three important features of a typing system: discrimination, typability and reproducibility.<\/jats:p>","DOI":"10.1017\/s0950268800068229","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T12:39:50Z","timestamp":1255955990000},"page":"289-295","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Non-radioactive ribotyping of<i>Haemophilus ducreyi<\/i>using a digoxigenin labelled cDNA probe"],"prefix":"10.1017","volume":"110","author":[{"given":"T. J.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. A.","family":"Ison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"S0950268800068229_ref012","first-page":"558","volume-title":"Berg's manual of systematic bacteriology","volume":"1","author":"Krieg","year":"1984"},{"key":"S0950268800068229_ref002","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.2.137"},{"key":"S0950268800068229_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF02097188"},{"key":"S0950268800068229_ref007","first-page":"123","article-title":"Typing Haemophilus ducreyi by indirect immunofluorescence assay","volume":"61","author":"Slootmans","year":"1985","journal-title":"Genitourin Med"},{"key":"S0950268800068229_ref010","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1128\/JCM.29.9.1949-1954.1991","article-title":"Molecular characterization of Haemophilus ducreyi by ribosoinal DNA fingerprinting","volume":"29","author":"Sarafian","year":"1991","journal-title":"J Clin Mierobiol"},{"key":"S0950268800068229_ref006","doi-asserted-by":"publisher","DOI":"10.1007\/BF01964253"},{"key":"S0950268800068229_ref001","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1128\/MMBR.53.4.377-389.1989","article-title":"Biology of Haemophilus ducreyi","volume":"53","author":"Albritton","year":"1989","journal-title":"Microbiol Rev"},{"key":"S0950268800068229_ref003","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.298.6674.623"},{"key":"S0950268800068229_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.4.710"},{"key":"S0950268800068229_ref008","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1128\/JCM.29.10.2333-2334.1991","article-title":"Novel plasmid combinations in Haemophilus ducreyi isolates from Thailand","volume":"29","author":"Sarafian","year":"1991","journal-title":"J Clin Microbiol"},{"key":"S0950268800068229_ref009","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.2.280"},{"key":"S0950268800068229_ref011","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02557.x"},{"key":"S0950268800068229_ref014","first-page":"2465","volume":"26","author":"Hunter","year":"1988","journal-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson's Index of Diversity"},{"key":"S0950268800068229_ref015","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-2-277"},{"key":"S0950268800068229_ref013","first-page":"17","volume-title":"Molecular cloning, a laboratory manual","author":"Sambrook","year":"1989"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800068229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T03:44:44Z","timestamp":1590205484000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800068229\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,4]]}},"alternative-id":["S0950268800068229"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800068229","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"type":"print","value":"0950-2688"},{"type":"electronic","value":"1469-4409"}],"subject":[],"published":{"date-parts":[[1993,4]]},"assertion":[{"value":"Cambridge University Press 1993","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}