{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:57:46Z","timestamp":1759010266024},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T00:00:00Z","timestamp":1255910400000},"content-version":"unspecified","delay-in-days":5436,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The genomic DNA of 39 strains of <jats:italic>Salmonella typhi<\/jats:italic> isolated from local residents and patients who had visited countries in the Asian region was analysed for restriction fragment length polymorphisms (RFLP). Pulsed-field gel electrophoretic (PFGE) analysis of <jats:italic>Xba<\/jats:italic> I- and <jats:italic>Spe<\/jats:italic> I-generated genomic restriction fragments established 22 PFGE types whereas phage typing differentiated the 39 isolates into 9 distinct phage types. This study showed that PFGE is more discriminatory than phage typing as it is capable of subtyping <jats:italic>S. typhi<\/jats:italic> strains of the same phage types. Genetic relatedness among the isolates was determined. Seven major clusters were identified at <jats:italic>SABS<\/jats:italic>of &gt; 0\u201380 and the remaining 13 isolates were distributed into minor clusters which were related at <jats:italic>SABS<\/jats:italic> of less than O.80. In conclusion, PFGE analysis in conjunction with distance matrix analysis served as a useful tool for delineating common <jats:italic>S. typhi<\/jats:italic> phage types of diverse origins from different geographical localesand separated in time.<\/jats:p>","DOI":"10.1017\/s0950268800068400","type":"journal-article","created":{"date-parts":[[2009,10,19]],"date-time":"2009-10-19T12:39:45Z","timestamp":1255955985000},"page":"391-402","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":32,"title":["Genome fingerprinting of <i>Salmonella typhi<\/i> by pulsed-field gel electrophoresis for subtyping common phage types"],"prefix":"10.1017","volume":"113","author":[{"given":"S.","family":"Nair","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L.","family":"Poh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. S.","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Tay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. T.","family":"Goh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"S0950268800068400_ref025","first-page":"1491","volume":"30","author":"Shoonmaker","year":"1992","journal-title":"Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during nosocomial outbreak"},{"key":"S0950268800068400_ref021","first-page":"454","volume":"39","author":"Ej","year":"1993","journal-title":"Insertion sequence IS200 can differentiate drugresistant and drug-sensitive Salmonella typhi of Vi-phage types El and Ml. J Med Microbiol"},{"key":"S0950268800068400_ref006","first-page":"398","volume":"32","author":"Yr","year":"1993","journal-title":"Use of pulsed-field gel electrophoresis for epidemiological study of Bordelella pertussis in a whooping cough outbreak"},{"key":"S0950268800068400_ref010","first-page":"94","volume":"9","author":"Es","year":"1956","journal-title":"Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology"},{"key":"S0950268800068400_ref026","first-page":"1135","volume":"32","author":"Kl","year":"1994","journal-title":"Epidemiologic analysis of sporadic Salmonella typhi isolates and those from outbreaks by pulsed-field gel electrophoresis"},{"key":"S0950268800068400_ref027","first-page":"7753","volume":"85","author":"Beltran","year":"1988","journal-title":"Toward a population genetic analysis of Salmonella: genetic diversity and relationships among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteriditis, S. heidelberg, S. infatis, S. newport and S. lyphimurium"},{"key":"S0950268800068400_ref011","first-page":"5840","volume":"171","author":"Suwanto","year":"1989","journal-title":"Physical and genetic mapping of the Ilhodobacter sphaeroides 2.4.1.genome: genome size, fragment identification and gene localization"},{"key":"S0950268800068400_ref003","first-page":"145","volume":"160","author":"Altwegg","year":"1989","journal-title":"Ribosomal RNA gene restriction patterns provide increased sensitivitv for tvping Salmonella typhi strains"},{"key":"S0950268800068400_ref028","first-page":"2262","volume":"8","author":"UK","year":"1990","journal-title":"Evolutionary genetic relationships of clones of Salmonella serovars that cause human typhoid and other enteric fevers"},{"key":"S0950268800068400_ref019","first-page":"565","volume":"107","author":"Natasi","year":"1991","journal-title":"RRNA fingerprinting as a tool in epidemiological analysis of Salmonella typhi infections"},{"key":"S0950268800068400_ref001","first-page":"329","volume":"8","author":"Edelman","year":"1986","journal-title":"Summary of an international workshop on typhoid fever"},{"key":"S0950268800068400_ref009","first-page":"523","volume":"138","author":"Ja","year":"1992","journal-title":"Analysis of tho clonal relationships between strains of Neisseria meningitidis by pulsed-field gel electrophoresis"},{"key":"S0950268800068400_ref022","first-page":"539","volume":"36","author":"Pang","year":"1992","journal-title":"Genetic variation among Malaysian isolates of Salmonella typhi as detected by ribosomal RNA gene restriction patterns"},{"key":"S0950268800068400_ref017","first-page":"202","volume":"17","author":"Boreoka","year":"1973","journal-title":"To the question of stability of Salmonella typhi phage types"},{"key":"S0950268800068400_ref007","first-page":"2599","volume":"30","author":"Mj","year":"1992","journal-title":"Epidemiology typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomie DNA by using pulsed-field gel electrophoresis"},{"key":"S0950268800068400_ref002","first-page":"172","volume":"17","author":"Fw","year":"1983","journal-title":"Regional centers for Salmonella typhi bacteriophage typing in the United States from 1974 through 1981"},{"key":"S0950268800068400_ref008","first-page":"2713","volume":"137","author":"We","year":"1991","journal-title":"Epidemiological analysis of a methicillin-resistant Staphylococcus aureus outbreak using restriction fragment length polymorphism of genomic DNA"},{"key":"S0950268800068400_ref016","first-page":"831","volume":"35","author":"Maher Ko'","year":"1986","journal-title":"Molecular techniques in the study of Salmonella lyphi in epidemiologic studies in endemic areas: comparison with Vi phage typing"},{"key":"S0950268800068400_ref013","first-page":"1236","volume":"28","author":"Schmid","year":"1990","journal-title":"Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3"},{"key":"S0950268800068400_ref014","first-page":"5269","volume":"76","author":"Nei","year":"1979","journal-title":"Mathematical model for studying genetic variation in terms of restriction endonucleases"},{"key":"S0950268800068400_ref018","first-page":"313","volume":"27","author":"Mw","year":"1989","journal-title":"Clonal nature of Salmonella typhi and its genetic relatedness to other Salmonella as shown by multilocus enzyme electrophoresis, and proposal of Salmonella bongori comb"},{"key":"S0950268800068400_ref020","first-page":"551","volume":"151","author":"Be","year":"1985","journal-title":"Survey of plasmids in Salmonella typhi from Chile and Thailand"},{"key":"S0950268800068400_ref012","first-page":"37","volume":"3","author":"Dr","year":"1983","journal-title":"DNA fingerprinting of Candida albicans"},{"key":"S0950268800068400_ref005","first-page":"2152","volume":"31","author":"Mj","year":"1983","journal-title":"Evaluation of the molecular epidemiology of an outbreak of multiple resistant Shigella sonnei in a day-care center by using pulsed-filed gel electrophoresis and plasmid DNA analysis"},{"key":"S0950268800068400_ref023","first-page":"817","volume":"11","author":"Cl","year":"1992","journal-title":"Genome fingerprinting by pulsed-field gel electrophoresis and ribotyping to differentiate Pseudomonas aeruginosa serotype 011 strains"},{"key":"S0950268800068400_ref024","first-page":"967","volume":"30","author":"Prevost","year":"1992","journal-title":"DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates"},{"key":"S0950268800068400_ref015","first-page":"3190","volume":"31","author":"Hellstein","year":"1993","journal-title":"Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity"},{"key":"S0950268800068400_ref004","first-page":"156","volume":"154","author":"Ls","year":"1986","journal-title":"Cloned random chromosomal sequences as probes to identify Salmonella species"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268800068400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T23:40:13Z","timestamp":1557877213000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268800068400\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,12]]}},"alternative-id":["S0950268800068400"],"URL":"https:\/\/doi.org\/10.1017\/s0950268800068400","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12]]},"assertion":[{"value":"Cambridge University Press 1994","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}