{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T05:43:14Z","timestamp":1782538994635,"version":"3.54.5"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2005,4,29]],"date-time":"2005-04-29T00:00:00Z","timestamp":1114732800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p>The purposes of the study were (i) to describe the abundance and epidemiology of Legionellaceae in the man-made environment in a northern Italian area, (ii) to assess the concordance between pulsed-field gel electrophoresis (PFGE) and automated ribotyping (AR) techniques for genotyping <jats:italic>L. pneumophila<\/jats:italic> and (iii) to investigate the correlation between serogrouping and genotyping data. Water was sampled from reservoirs in 12 buildings across an area of 80-km radius. Despite the water temperature always being maintained above 55 \u00b0C, all of the buildings sampled were contaminated with Legionellaceae on at least one occasion and 63 <jats:italic>L. pneumophila<\/jats:italic> isolates representing nine different serogroups were collected. The two DNA methods revealed a high degree of genetic heterogeneity, even though identical <jats:italic>L. pneumophila<\/jats:italic> clones were recovered at different sites. The AR technique provided a fairly reliable approximation of PFGE results (73% concordance), however there was poor correlation between serogrouping and genotyping data as identical DNA fingerprints were shared by isolates of different serogroups.<\/jats:p>","DOI":"10.1017\/s0950268805004395","type":"journal-article","created":{"date-parts":[[2005,11,8]],"date-time":"2005-11-08T11:42:55Z","timestamp":1131450175000},"page":"1097-1105","source":"Crossref","is-referenced-by-count":7,"title":["Molecular epidemiology of <i>Legionella pneumophila<\/i> environmental isolates representing nine different serogroups determined by automated ribotyping and pulsed-field gel electrophoresis"],"prefix":"10.1017","volume":"133","author":[{"given":"S.","family":"BOCCIA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"STENICO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"AMORE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"MORODER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"ORSINI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"ROMANO-SPICA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"RICCIARDI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2005,4,29]]},"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268805004395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T07:25:33Z","timestamp":1750490733000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268805004395\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,29]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["S0950268805004395"],"URL":"https:\/\/doi.org\/10.1017\/s0950268805004395","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,29]]}}}