{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:24:57Z","timestamp":1784309097440,"version":"3.55.0"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2007,1,15]],"date-time":"2007-01-15T00:00:00Z","timestamp":1168819200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>We determined the incidence of cryptosporidiosis in children aged &lt;5 years presenting with diarrhoea in an urban and rural hospital-based setting in Malawi. Stools were collected over a 22-month period during both rainy and dry seasons. A range of microscopic methods were used to determine the presence of<jats:italic>Cryptosporidium<\/jats:italic>spp. oocysts. Species determination was by polymerase chain reaction\u2013restriction fragment length polymorphism (PCR\u2013RFLP) of oocyst-extracted DNA using 18S rRNA and COWP gene loci.<jats:italic>Cryptosporidium<\/jats:italic>spp. oocysts were seen in 5\u00b79% (50\/848) of samples, of which 43 amplified by PCR\u2013RFLP indicated the following species:<jats:italic>C. hominis<\/jats:italic>,<jats:italic>C. parvum<\/jats:italic>,<jats:italic>C. hominis\/C. parvum<\/jats:italic>,<jats:italic>C. meleagridis<\/jats:italic>and<jats:italic>C. andersoni<\/jats:italic>. Seven samples could not be amplified by PCR. Wider species diversity was found in the rural setting, and may be a result of increased malnutrition and zoonotic exposure in this area. Improvements in water, sanitation, household hygiene and animal control are required to reduce the incidence of infection in this population.<\/jats:p>","DOI":"10.1017\/s0950268806007758","type":"journal-article","created":{"date-parts":[[2007,1,15]],"date-time":"2007-01-15T18:28:48Z","timestamp":1168885728000},"page":"1307-1315","source":"Crossref","is-referenced-by-count":91,"title":["Incidence of cryptosporidiosis species in paediatric patients in Malawi"],"prefix":"10.1017","volume":"135","author":[{"given":"T.\u00a0D.","family":"MORSE","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.\u00a0A.\u00a0B.","family":"NICHOLS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.\u00a0M.","family":"GRIMASON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.\u00a0M.","family":"CAMPBELL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.\u00a0C.","family":"TEMBO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u00a0V.","family":"SMITH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2007,1,15]]},"reference":[{"key":"S0950268806007758_ref006","doi-asserted-by":"publisher","DOI":"10.1080\/02724936.1990.11747402"},{"key":"S0950268806007758_ref034","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.2002.01220.x"},{"key":"S0950268806007758_ref037","doi-asserted-by":"publisher","DOI":"10.1053\/jinf.2001.0839"},{"key":"S0950268806007758_ref002","unstructured":"2.\u00a0National Statistical Office (Malawi). 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