{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:17:32Z","timestamp":1758709052015},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2008,6,9]],"date-time":"2008-06-09T00:00:00Z","timestamp":1212969600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The cytopathogenicity of 22<jats:italic>Legionella pneumophila<\/jats:italic>isolates from 17 hospitals was determined by assessing the dose of bacteria necessary to produce 50% cytopathic effect (CPED<jats:sub>50<\/jats:sub>) in U937 human-derived macrophages. All isolates were able to infect and grow in macrophage-like cells (range log<jats:sub>10<\/jats:sub>CPED<jats:sub>50<\/jats:sub>: 2\u00b767\u20136\u00b773 c.f.u.\/ml). Five groups were established and related to the serogroup, the number of PFGE patterns coexisting in the same hospital water distribution system, and the possible reporting of hospital-acquired Legionnaires' disease cases.<jats:italic>L. pneumophila<\/jats:italic>serogroup 1 isolates had the highest cytopathogenicity (<jats:italic>P<\/jats:italic>=0\u00b7003). Moreover, a trend to more cytopathogenic groups (groups 1\u20133) in hospitals with more than one PFGE pattern of<jats:italic>L. pneumophila<\/jats:italic>in the water distribution system (60%<jats:italic>vs<\/jats:italic>. 17%) and in hospitals reporting cases of hospital-acquired Legionnaires' disease (36\u00b73%<jats:italic>vs<\/jats:italic>. 16\u00b76%) was observed. We conclude that the cytopathogenicty of environmental<jats:italic>L. pneumophila<\/jats:italic>should be taken into account in evaluating the risk of a contaminated water reservoir in a hospital and hospital acquisition of Legionnaires' disease.<\/jats:p>","DOI":"10.1017\/s0950268808000691","type":"journal-article","created":{"date-parts":[[2008,6,9]],"date-time":"2008-06-09T08:28:13Z","timestamp":1213000093000},"page":"188-193","source":"Crossref","is-referenced-by-count":7,"title":["Cytopathogenicity and molecular subtyping of<i>Legionella pneumophila<\/i>environmental isolates from 17 hospitals"],"prefix":"10.1017","volume":"137","author":[{"given":"M.","family":"GARCIA-NU\u00d1EZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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