{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:13:14Z","timestamp":1791007994766,"version":"4.1.0"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2009,1,27]],"date-time":"2009-01-27T00:00:00Z","timestamp":1233014400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Rift Valley fever virus (RVFV) is a mosquito-transmitted bunyavirus (genus<jats:italic>Phlebovirus<\/jats:italic>) associated with severe disease in livestock and fatal encephalitis or haemorrhagic fever in a proportion of infected humans. Although live attenuated and inactivated vaccines have been used in livestock, and on a limited scale in humans, there is a need for improved anti-RVFV vaccines. Towards this goal, Sindbis virus replicon vectors expressing the RVFV Gn and Gc glycoproteins, as well as the non-structural nsM protein, were constructed and evaluated for their ability to induce protective immune responses against RVFV. These replicon vectors were shown to produce the RVFV glycoproteins to high levels<jats:italic>in vitro<\/jats:italic>and to induce systemic anti-RVFV antibody responses in immunized mice, as determined by RVFV-specific ELISA, fluorescent antibody tests, and demonstration of a neutralizing antibody response. Replicon vaccination also provided 100% protection against lethal RVFV challenge by either the intraperitoneal or intranasal route. Furthermore, preliminary results indicate that the replicon vectors elicit RVFV-specific neutralizing antibody responses in vaccinated sheep. These results suggest that alphavirus-based replicon vectors can induce protective immunity against RVFV, and that this approach merits further investigation into its potential utility as a RVFV vaccine.<\/jats:p>","DOI":"10.1017\/s0950268808001696","type":"journal-article","created":{"date-parts":[[2009,1,27]],"date-time":"2009-01-27T09:43:47Z","timestamp":1233049427000},"page":"1309-1318","source":"Crossref","is-referenced-by-count":47,"title":["An alphavirus replicon-derived candidate vaccine against Rift Valley fever virus"],"prefix":"10.1017","volume":"137","author":[{"given":"M. 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