{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:52:44Z","timestamp":1775152364450,"version":"3.50.1"},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,9,18]],"date-time":"2009-09-18T00:00:00Z","timestamp":1253232000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Most of the multiplex PCR (mPCR) used to identify<jats:italic>Shigella<\/jats:italic>do not discriminate between<jats:italic>Shigella<\/jats:italic>species or serotypes. We designed a mPCR to differentiate between<jats:italic>S. flexneri<\/jats:italic>and<jats:italic>S. sonnei<\/jats:italic>strains based on the detection of markers associated with the<jats:italic>she<\/jats:italic>pathogenicity island described in<jats:italic>Shigella<\/jats:italic>. In addition, specific primers were included to detect the<jats:italic>Shigella<\/jats:italic>virulence determinants ShET-1 and ShET-2 enterotoxin genes. The analysis of 304<jats:italic>Shigella<\/jats:italic>strains from Chile and 79<jats:italic>Shigella<\/jats:italic>strains from other geographic locations indicated that the mPCR described here detected all<jats:italic>Shigella<\/jats:italic>species and specifically differentiated<jats:italic>S. flexneri<\/jats:italic>and<jats:italic>S. sonnei<\/jats:italic>. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes.<\/jats:p>","DOI":"10.1017\/s0950268809990823","type":"journal-article","created":{"date-parts":[[2009,9,18]],"date-time":"2009-09-18T14:55:31Z","timestamp":1253285731000},"page":"525-533","source":"Crossref","is-referenced-by-count":24,"title":["A new multiplex PCR for differential identification of<i>Shigella flexneri<\/i>and<i>Shigella sonnei<\/i>and detection of<i>Shigella<\/i>virulence determinants"],"prefix":"10.1017","volume":"138","author":[{"given":"M. J.","family":"FARF\u00c1N","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. A.","family":"GARAY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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