{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:17:16Z","timestamp":1773299836051,"version":"3.50.1"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T00:00:00Z","timestamp":1325808000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The assemblages of <jats:italic>Giardia duodenalis<\/jats:italic> in 25 children with and 25 children without diarrhoea and 24 adults with gastrointestinal symptoms in South India were determined. Polymerase chain reaction\u2013restriction fragment length polymorphism (PCR\u2013RFLP) targeting the glutamate dehydrogenase (<jats:italic>gdh<\/jats:italic>), \u03b2<jats:italic>-giardin<\/jats:italic> and triosephosphate isomerase (<jats:italic>tpi<\/jats:italic>) genes was used. The <jats:italic>tpi<\/jats:italic> PCR was the most sensitive and detected <jats:italic>G. duodenalis<\/jats:italic> in all 74 microscopy-positive samples, while <jats:italic>gdh<\/jats:italic> and \u03b2<jats:italic>-giardin<\/jats:italic> PCR were positive in 62\u00b72% and 56\u00b78% of the samples. Assemblage B was predominant in both children and adults (82\u00b74%) followed by assemblage AII (9\u00b74%); assemblage AI was not detected. Infections with both assemblages A and B (detected by <jats:italic>tpi<\/jats:italic> PCR\u2013RFLP) were seen exclusively in children and the mixed assemblage BIII and BIV (detected by <jats:italic>gdh<\/jats:italic> PCR\u2013RFLP) was more common in children than adults (<jats:italic>P<\/jats:italic>=0\u00b7058).<\/jats:p>","DOI":"10.1017\/s0950268811002767","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T13:47:29Z","timestamp":1325857649000},"page":"2023-2027","source":"Crossref","is-referenced-by-count":14,"title":["Mixed <i>Giardia duodenalis<\/i> assemblage infections in children and adults in South India"],"prefix":"10.1017","volume":"140","author":[{"given":"S.","family":"LAISHRAM","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"KANNAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"RAJENDRAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"KANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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