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Infect."],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Due to the poor positive predictive value of nucleic acid amplification tests (NAATs) for gonorrhoea when applied to a low-prevalence setting, current guidelines recommend the use of supplementary polymerase chain reaction (PCR) targeting a different gene for confirmation of true positives in urogenital specimens. This study sought to standardize and evaluate performance of an in-house<jats:italic>opa<\/jats:italic>gene-based PCR assay for gonorrhoea compared to assays targeting the<jats:italic>porA<\/jats:italic>pseudogene and 16S rRNA gene. Four hundred samples (300 endocervical, 100 urethral swabs) from patients attending STD clinics in New Delhi, India were used. The sensitivity, specificity, positive predictive value and negative predictive value of the<jats:italic>opa<\/jats:italic>-based PCR were 100%, 97\u00b79%, 89\u00b75% and 100%, respectively. In females, the use of NAATs provided enhanced diagnosis of gonorrhoea.<\/jats:p>","DOI":"10.1017\/s0950268811002883","type":"journal-article","created":{"date-parts":[[2012,1,16]],"date-time":"2012-01-16T11:48:37Z","timestamp":1326714517000},"page":"2110-2116","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of an<i>opa<\/i>gene-based nucleic acid amplification test for detection of<i>Neisseria gonorrhoeae<\/i>in urogenital samples in North India"],"prefix":"10.1017","volume":"140","author":[{"given":"R.","family":"VERMA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"SOOD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"BALA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"MAHAJAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"KAPIL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. 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Accessed 17 November 2011."},{"key":"S0950268811002883_ref017","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01624-10"},{"key":"S0950268811002883_ref002","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1128\/JCM.33.11.3049-3053.1995","article-title":"Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in genitourinary specimens","volume":"33","author":"Mahony","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268811002883_ref004","first-page":"358","article-title":"Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network","volume":"29","author":"Smith","year":"2005","journal-title":"Communicable Diseases Intelligence"},{"key":"S0950268811002883_ref016","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00834-06"},{"key":"S0950268811002883_ref003","doi-asserted-by":"publisher","DOI":"10.1136\/sti.77.3.153"},{"key":"S0950268811002883_ref005","doi-asserted-by":"publisher","DOI":"10.1086\/381895"},{"key":"S0950268811002883_ref020","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000154495.24519.bf"},{"key":"S0950268811002883_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.12.007"},{"key":"S0950268811002883_ref001","unstructured":"1. WHO. Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates. Geneva, Switzerland: World Health Organization, 2001."},{"key":"S0950268811002883_ref015","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2231-2235.2005"},{"key":"S0950268811002883_ref018","doi-asserted-by":"crossref","DOI":"10.2807\/ese.16.21.19874-en","article-title":"False-negative results using Neisseria gonorrhoeae porA pseudogene PCR \u2013 a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011","volume":"16","author":"Whiley","year":"2011","journal-title":"Eurosurveillance"},{"key":"S0950268811002883_ref010","unstructured":"10. WHO. Laboratory diagnosis of gonorrhoea. WHO Regional Publication, South East Asia series no. 33. 1999. Geneva: World Health Organization."},{"key":"S0950268811002883_ref014","doi-asserted-by":"publisher","DOI":"10.1080\/00313020600928253"},{"key":"S0950268811002883_ref007","first-page":"1","article-title":"Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections: 2002","volume":"51","author":"Johnson","year":"2002","journal-title":"Morbidity and Mortality Weekly Reports"},{"key":"S0950268811002883_ref006","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01217-11"},{"key":"S0950268811002883_ref011","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.060024"},{"key":"S0950268811002883_ref009","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.041418"},{"key":"S0950268811002883_ref012","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1128\/JCM.37.2.386-390.1999","article-title":"Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR","volume":"37","author":"Farrell","year":"1999","journal-title":"Journal of Clinical Microbiology"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268811002883","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T04:33:20Z","timestamp":1640493200000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268811002883\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,16]]},"references-count":21,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["S0950268811002883"],"URL":"https:\/\/doi.org\/10.1017\/s0950268811002883","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,16]]}}}