{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:11:16Z","timestamp":1793344276240,"version":"4.5.0"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T00:00:00Z","timestamp":1346976000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    We report an evaluation of the accuracy of ELISA for the detection of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    -specific antibodies in humans. Eighty-eight studies published in 35 articles met all inclusion criteria and were submitted to meta-analysis. Pooled sensitivity and specificity were 0\u00b7779 (95% CI 0\u00b7770\u20130\u00b7789) and 0\u00b7913 (95% CI 0\u00b7908\u20130\u00b7917), respectively, and the area under the curve was 0\u00b7964. Heterogeneity across studies was statistically significant, but none of the sources of heterogeneity (disease stage, antigen used, antibody detected) could fully explain this finding. Although the convalescent stage of disease was significantly associated with higher diagnostic accuracy, IgM ELISA was the best choice, regardless of the stage of disease. Negative ELISAs (IgG or IgM) applied in the acute phase do not rule out leptospirosis due to the possibility of false-negative results. In this case it is advisable to request a second blood sample or to apply a direct method for leptospiral DNA.\n                  <\/jats:p>","DOI":"10.1017\/s0950268812001951","type":"journal-article","created":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T05:38:08Z","timestamp":1346996288000},"page":"22-32","source":"Crossref","is-referenced-by-count":51,"title":["Enzyme-linked immunosorbent assay to diagnose human leptospirosis: a meta-analysis of the published literature"],"prefix":"10.1017","volume":"141","author":[{"given":"M. L.","family":"SIGNORINI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"LOTTERSBERGER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. D.","family":"TARABLA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. B.","family":"VANASCO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2012,9,7]]},"reference":[{"key":"S0950268812001951_ref45","unstructured":"Vanasco NB , Evaluation of ELISA antigens for leptospiral serodiagnosis in different stages of the disease. VII Reuni\u00f3n de la Sociedad Internacional de Leptospirosis, Merida, Yucatan, Mexico, 19\u201322 September 2011. Oral presentation."},{"key":"S0950268812001951_ref40","first-page":"172","article-title":"Diagnosis of leptospirosis by recombinant antigen based single serum dilution ELISA","volume":"128","author":"Dey","year":"2008","journal-title":"Indian Journal of Medical Research"},{"key":"S0950268812001951_ref39","first-page":"309","article-title":"Evaluation an in-house IgM-ELISA for the diagnosis of human leptospirosis","volume":"9","author":"Honarmand","year":"2008","journal-title":"Journal of Semnan University of Medical Sciences"},{"key":"S0950268812001951_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.10.012"},{"key":"S0950268812001951_ref36","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00217-07"},{"key":"S0950268812001951_ref35","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00219-06"},{"key":"S0950268812001951_ref34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2006.01727.x"},{"key":"S0950268812001951_ref33","first-page":"302","article-title":"Two simple immunoassays using endemic leptospiral antigens for serodiagnosis of human leptospirosis","volume":"36","author":"Tansuphasiri","year":"2005","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0950268812001951_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.803-809.2003"},{"key":"S0950268812001951_ref29","first-page":"1","article-title":"Enzyme-linked immunosorbent assay for the detection of IgM in the diagnostic of human leptospirosis","volume":"19","author":"C\u00e9spedes","year":"2002","journal-title":"Revista Peruana de Medicina Experimental y Salud Publica"},{"key":"S0950268812001951_ref17","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651992000300010"},{"key":"S0950268812001951_ref15","first-page":"400","article-title":"Serodiagnosis of human leptospirosis by enzyme-linked-immunosorbent-assay (ELISA)","volume":"247","author":"Terpstra","year":"1980","journal-title":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. 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VII ILS Meeting, Merida, Mexico, 19\u201322 September, 2011."}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268812001951","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T12:29:17Z","timestamp":1687696157000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268812001951\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,7]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["S0950268812001951"],"URL":"https:\/\/doi.org\/10.1017\/s0950268812001951","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,7]]}}}