{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T16:09:09Z","timestamp":1785600549384,"version":"3.56.0"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T00:00:00Z","timestamp":1355097600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>We investigated the virulence gene carriage and molecular type characteristics of<jats:italic>Staphylococcus aureus<\/jats:italic>isolates from bloodstream infections (BSIs) and skin and soft tissue infections (SSTIs) in children. A total of 71 isolates, 16 of which were methicillin-resistant<jats:italic>S. aureus<\/jats:italic>(MRSA), were investigated by PCR for virulence-associated gene profiles, sequence type and<jats:italic>spa<\/jats:italic>type. This revealed that 76\u00b77% and 53\u00b77% of the SSTI and BSI isolates, respectively, exhibited simultaneous carriage of \u2a7e10 virulence genes. Compared to BSI isolates, carriage rates for<jats:italic>hla<\/jats:italic>,<jats:italic>hlb<\/jats:italic>,<jats:italic>cna<\/jats:italic>,<jats:italic>clfA<\/jats:italic>,<jats:italic>seb<\/jats:italic>,<jats:italic>sec<\/jats:italic>and<jats:italic>pvl<\/jats:italic>genes were significantly higher in SSTI isolates. By contrast, carriage of<jats:italic>eta<\/jats:italic>,<jats:italic>etb<\/jats:italic>and<jats:italic>sea<\/jats:italic>was significantly higher for BSI isolates. Thirty-four sequence types (STs) and 36<jats:italic>spa<\/jats:italic>types were identified in the 71 isolates and included 14 novel STs and four novel<jats:italic>spa<\/jats:italic>types. ST59-MRSA-IV\/V-t437 was the most common clone in the MRSA isolates. We concluded that virulence determinants are widely distributed in isolates of<jats:italic>S. aureus<\/jats:italic>strains from children with BSIs and SSTIs, with an unexpectedly high rate in SSTI isolates. Future profiling of<jats:italic>S. aureus<\/jats:italic>virulence determinants may allow the prediction of severity and outcome for children with these infections.<\/jats:p>","DOI":"10.1017\/s0950268812002919","type":"journal-article","created":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T10:55:38Z","timestamp":1355136938000},"page":"2158-2162","source":"Crossref","is-referenced-by-count":12,"title":["Carriage of virulence factors and molecular characteristics of<i>Staphylococcus aureus<\/i>isolates associated with bloodstream, and skin and soft tissue infections in 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