{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:59:03Z","timestamp":1718845143310},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T00:00:00Z","timestamp":1374105600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The presence of 10 virulence genes was examined using polymerase chain reaction (PCR) in 365 European O157 and non-O157<jats:italic>Escherichia coli<\/jats:italic>isolates associated with verotoxin production. Strain-specific PCR data were analysed using hierarchical clustering. The resulting dendrogram clearly separated O157 from non-O157 strains. The former clustered typical high-risk seropathotype (SPT) A strains from all regions, including Sweden and Spain, which were homogenous by Cramer's<jats:italic>V<\/jats:italic>statistic, and strains with less typical O157 features mostly from Hungary. The non-O157 strains divided into a high-risk SPTB harbouring O26, O111 and O103 strains, a group pathogenic to pigs, and a group with few virulence genes other than for verotoxin. The data demonstrate SPT designation and selected PCR separated verotoxigenic<jats:italic>E. coli<\/jats:italic>of high and low risk to humans; although more virulence genes or pulsed-field gel electrophoresis will need to be included to separate high-risk strains further for epidemiological tracing.<\/jats:p>","DOI":"10.1017\/s0950268813001635","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T14:07:20Z","timestamp":1374156440000},"page":"1019-1028","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Use of virulence determinants and seropathotypes to distinguish high- and low-risk<i>Escherichia coli<\/i>O157 and non-O157 isolates from Europe"],"prefix":"10.1017","volume":"142","author":[{"given":"M. F.","family":"ANJUM","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"JONES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"MORRISON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"TOZZOLI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"MORABITO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"TOTH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"NAGY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"SMITH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"ASPAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"NIELSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"FACH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"HERRERA-LE\u00d3N","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"WOODWARD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"LA RAGIONE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,7,18]]},"reference":[{"key":"S0950268813001635_ref12","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d915"},{"key":"S0950268813001635_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00873-09"},{"key":"S0950268813001635_ref4","unstructured":"European Centre for Disease Control and Prevention. ECDC surveillance report, Quarterly STEC\/VTEC Report Q1 2008, January\u2013March 2008."},{"key":"S0950268813001635_ref3","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.1.15"},{"key":"S0950268813001635_ref11","doi-asserted-by":"publisher","DOI":"10.1136\/vr.c4009"},{"key":"S0950268813001635_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02566-07"},{"key":"S0950268813001635_ref29","doi-asserted-by":"publisher","DOI":"10.1038\/35054089"},{"key":"S0950268813001635_ref5","unstructured":"World Health Organisation. Zoonotic non-O157 shiga toxin-producing Escherichia coli (STEC). Report of a WHO Scientific Working Group Meeting, Berlin, 23\u201326 June 1998."},{"key":"S0950268813001635_ref9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.4930-4940.2003"},{"key":"S0950268813001635_ref22","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00512-10"},{"key":"S0950268813001635_ref34","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00816-08"},{"key":"S0950268813001635_ref27","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.546-550.2005"},{"key":"S0950268813001635_ref18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09311.x"},{"key":"S0950268813001635_ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04460.x"},{"key":"S0950268813001635_ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2004.apm1120903.x"},{"key":"S0950268813001635_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00218-1"},{"key":"S0950268813001635_ref32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903585106"},{"key":"S0950268813001635_ref28","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.17.4690-4698.2002"},{"key":"S0950268813001635_ref24","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/JCM.34.12.2980-2984.1996","article-title":"Shiga toxin-producing Escherichia coli strains from bovines: association of adhesion with carriage of eae and other genes","volume":"34","author":"Wieler","year":"1996","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268813001635_ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022751"},{"key":"S0950268813001635_ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04887.x"},{"key":"S0950268813001635_ref23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-166"},{"key":"S0950268813001635_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.02.007"},{"key":"S0950268813001635_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01921-09"},{"key":"S0950268813001635_ref21","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3343-3348.2003"},{"key":"S0950268813001635_ref15","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.8.1556"},{"key":"S0950268813001635_ref7","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02322-10"},{"key":"S0950268813001635_ref2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036079"},{"key":"S0950268813001635_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00150-6"},{"key":"S0950268813001635_ref25","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-42-6-404"},{"key":"S0950268813001635_ref16","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0614"},{"key":"S0950268813001635_ref26","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5715-5720.2005"},{"key":"S0950268813001635_ref1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrheagenic Escherichia coli","volume":"11","author":"Nataro","year":"1998","journal-title":"Clinical Microbiology Reviews"},{"key":"S0950268813001635_ref33","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00419-07"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268813001635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T04:19:40Z","timestamp":1596169180000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268813001635\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,18]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["S0950268813001635"],"URL":"https:\/\/doi.org\/10.1017\/s0950268813001635","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,18]]},"assertion":[{"value":"Copyright \u00a9 Crown Copyright. Published by Cambridge University Press 2013\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}