{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:46:51Z","timestamp":1792428411286,"version":"4.5.0"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T00:00:00Z","timestamp":1409788800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A peptide library was used to screen for regions containing potential linear B-cell epitope sites in the glycoproteins and nucleoprotein of Crimean-Congo haemorrhagic fever virus (CCHFV) in an enzyme-linked immunosorbent assay (ELISA). The library consisted of 156 peptides, spanning the nucleoprotein and mature G<jats:sub>N<\/jats:sub>and G<jats:sub>C<\/jats:sub>proteins in a 19-mer with 9-mer overlap format. Using pooled serum samples from convalescent patients to screen the library, six peptides were identified as potential epitope sites. Further testing of these six peptides with individual patient sera identified two of these peptides as probable epitope sites, with peptide G<jats:sub>1451\u20131469<\/jats:sub>reacting to 13\/15 and peptide G<jats:sub>1613\u20131631<\/jats:sub>to 14\/15 human sera. These peptides are situated on the G<jats:sub>C<\/jats:sub>protein at amino acid positions 1451\u20131469 (relative to CCHFV isolate SPU103\/97) (TCTGCYACSSGISCKVRIH) and 1613\u20131631 (FMFGWRILFCFKCCRRTRG). Identified peptides may have application in ELISA for diagnostic or serosurveillance purposes.<\/jats:p>","DOI":"10.1017\/s0950268814002271","type":"journal-article","created":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T22:08:56Z","timestamp":1409868536000},"page":"1451-1456","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Identification of human linear B-cell epitope sites on the envelope glycoproteins of Crimean-Congo haemorrhagic fever virus"],"prefix":"10.1017","volume":"143","author":[{"given":"D.","family":"GOEDHALS","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. T.","family":"PAWESKA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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