{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:58:21Z","timestamp":1790812701252,"version":"4.1.0"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T00:00:00Z","timestamp":1419206400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>During a 4-year (2007\u20132010) survey, the presence of <jats:italic>Brucella suis<\/jats:italic> infection in domestic pigs in Sardinia was investigated. Serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the Rose Bengal (RBT) and complement fixation (CFT) tests for screening and confirmation of <jats:italic>Brucella<\/jats:italic>, respectively. Of the 1251 serum samples analysed by RBT, 406 sera, originating from 36 farms, were positive for <jats:italic>B. suis<\/jats:italic>. CFT was positive in 292\/748 sera analysed, confirming positivity in all 36 pig herds. Pigs with international complement fixation test units per ml (ICFTU\/ml) values \u2a7e160 were slaughtered, and their organs collected for bacteriological examination and testing by polymerase chain reaction (PCR). <jats:italic>Brucella<\/jats:italic> spp. strains were isolated in culture from 13\/502 organs analysed, and subsequently identified as <jats:italic>B. suis<\/jats:italic> biovar 2. PCR detected positivity to <jats:italic>Brucella<\/jats:italic> spp. in 19\/285 organs analysed. These results confirm the presence and emergence of <jats:italic>B. suis<\/jats:italic> infection in domestic pigs in Sardinia.<\/jats:p>","DOI":"10.1017\/s0950268814003513","type":"journal-article","created":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T11:18:57Z","timestamp":1419247137000},"page":"2170-2177","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":18,"title":["<i>Brucella suis<\/i> infection in domestic pigs in Sardinia (Italy)"],"prefix":"10.1017","volume":"143","author":[{"given":"C.","family":"PILO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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Terrestrial Manual of Standards for Diagnostic Tests and Vaccines, 2009, 6th edn. Chapter 2\u00b74\u00b73: Bovine brucellosis."},{"key":"S0950268814003513_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.04.003"},{"key":"S0950268814003513_ref12","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-45.4.1178"},{"key":"S0950268814003513_ref1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.160.11.362"},{"key":"S0950268814003513_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.02.056"},{"key":"S0950268814003513_ref22","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-116"},{"key":"S0950268814003513_ref18","doi-asserted-by":"publisher","DOI":"10.20506\/rst.28.3.1947"},{"key":"S0950268814003513_ref10","article-title":"Council Directive 97\/12\/EC of 12 March 1997 amending and updating Directive 64\/432\/ECC on health problems affecting intra-Community trade in bovine animals and swine","volume":"L 109","year":"1997","journal-title":"Official Journal of the European Communities"},{"key":"S0950268814003513_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2012.10.030"},{"key":"S0950268814003513_ref17","unstructured":"Italian Ministry of Health. Ministerial Decree of 20 April 1998: \u2018Declaration of territory officially free from bovine and bubaline brucellosis for the provinces of Cagliari, Oristano, Nuoro e Sassari, and for the Sardinia region\u2019 [in Italian]. Gazzetta Ufficiale della Repubblica Italiana, Serie Generale, n. 168 del 21 luglio 1998."},{"key":"S0950268814003513_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.055"},{"key":"S0950268814003513_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-012-0108-2"},{"key":"S0950268814003513_ref7","first-page":"618","article-title":"Brucella suis infection associated with feral swine hunting\u00a0\u2013\u00a0three states, 2007\u20132008","volume":"58","year":"2009","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"S0950268814003513_ref20","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-80"},{"key":"S0950268814003513_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2013.06.001"},{"key":"S0950268814003513_ref5","first-page":"1","article-title":"Scientific Opinion of the Panel on Animal Health and Welfare (AHAW) on a request from the Commission on porcine brucellosis (Brucella suis)","volume":"1144","year":"2009","journal-title":"EFSA Journal"},{"key":"S0950268814003513_ref13","first-page":"397","article-title":"Isolation of Brucella suis biovar 2 from a wild boar in the Abruzzo Region of Italy","volume":"48","author":"De","year":"2012","journal-title":"Veterinaria Italiana"},{"key":"S0950268814003513_ref16","unstructured":"Addis G , Seroprevalence of Brucella spp. in wild boars hunted during the shooting season of the years 2009\u20132010 in southern Sardinia [in Italian]. In: Proceedings of the 12th National Congress of the Italian Association of Veterinary Laboratory Diagnosticians. Genoa: Italian Association of Veterinary Laboratory Diagnosticians (S.I.Di.L.V), 2010, pp. 126\u2013127."},{"key":"S0950268814003513_ref11","first-page":"77","article-title":"Brucellosis in wild boars in the Piedmont Region","volume":"45","author":"Gennero","year":"2004","journal-title":"Epid\u00e9miologie et Sant\u00e9 Animale"},{"key":"S0950268814003513_ref19","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-39.3.718"},{"key":"S0950268814003513_ref15","unstructured":"OIE, World Organization for Animal Health. Terrestrial Manual of Standards for Diagnostic Tests and Vaccines, 2009, 6th edn. Chapter 2\u00b78\u00b75: Porcine brucellosis."},{"key":"S0950268814003513_ref21","unstructured":"Garin-Bastuji B , Is brucellosis due the biovar 2 of Brucella suis an emerging zoonosis in France? Two case reports in wild boar and hare hunters. In: Proceedings of the International Society of Chemotherapy Disease Management Meeting, 1st International Meeting on Treatment of Human Brucellosis, 2006, Ioannina, Greece."}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268814003513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,20]],"date-time":"2019-04-20T23:29:00Z","timestamp":1555802940000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268814003513\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,22]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["S0950268814003513"],"URL":"https:\/\/doi.org\/10.1017\/s0950268814003513","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,22]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2014\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}