{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:38:33Z","timestamp":1757702313379},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Orthobunyaviruses, tri-segmented, negative-sense RNA viruses, have long been associated with mild to severe human disease in Africa, but not haemorrhagic fever. However, during a Rift Valley fever outbreak in East Africa in 1997\u20131998, Ngari virus was isolated from two patients and antibody detected in several others with haemorrhagic fever. The isolates were used to identify Ngari virus as a natural <jats:italic>Orthobunyavirus<\/jats:italic> reassortant. Despite their potential to reassort and cause severe human disease, characterization of orthobunyaviruses is hampered by paucity of genetic sequences. Our objective was to obtain complete gene sequences of two Bunyamwera virus and three Ngari virus isolates from recent surveys in Kenya and to determine their phylogenetic positioning within the Bunyamwera serogroup. Newly sequenced Kenyan Bunyamwera virus isolates clustered closest to a Bunyamwera virus isolate from the same locality and a Central African Republic isolate indicating that similar strains may be circulating regionally. Recent Kenyan Ngari isolates were closest to the Ngari isolates associated with the 1997\u20131998 haemorrhagic fever outbreak. We observed a temporal\/geographical relationship among Ngari isolates in all three gene segments suggesting a geographical\/temporal association with genetic diversity. These sequences in addition to earlier sequences can be used for future analyses of this neglected but potentially deadly group of viruses.<\/jats:p>","DOI":"10.1017\/s0950268815001338","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T03:41:15Z","timestamp":1435549275000},"page":"389-395","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus <i>Orthobunyavirus<\/i>) isolated in Kenya"],"prefix":"10.1017","volume":"144","author":[{"given":"C.","family":"ODHIAMBO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"VENTER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"LWANDE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"SWANEPOEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"SANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2015,6,29]]},"reference":[{"key":"S0950268815001338_ref12","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Standley","year":"2013","journal-title":"Molecular Biology and Evolution"},{"key":"S0950268815001338_ref4","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1201"},{"key":"S0950268815001338_ref5","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02448-05"},{"key":"S0950268815001338_ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-006-0808-x"},{"key":"S0950268815001338_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.16.8922-8926.2004"},{"key":"S0950268815001338_ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-140"},{"key":"S0950268815001338_ref10","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.10-0483"},{"key":"S0950268815001338_ref7","doi-asserted-by":"publisher","DOI":"10.1603\/033.046.0431"},{"key":"S0950268815001338_ref3","first-page":"87","volume-title":"The Arboviruses: Epidemiology and Ecology","author":"Gonzalez","year":"1988"},{"key":"S0950268815001338_ref1","first-page":"1","volume-title":"The Bunyaviridae, the Viruses","author":"Calisher","year":"1996"},{"key":"S0950268815001338_ref14","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.051359-0"},{"key":"S0950268815001338_ref2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280591002496"},{"key":"S0950268815001338_ref8","doi-asserted-by":"publisher","DOI":"10.1603\/ME13039"},{"key":"S0950268815001338_ref13","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268815001338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,19]],"date-time":"2019-04-19T20:59:35Z","timestamp":1555707575000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268815001338\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,29]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["S0950268815001338"],"URL":"https:\/\/doi.org\/10.1017\/s0950268815001338","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,29]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2015\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}