{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:27:33Z","timestamp":1775741253039,"version":"3.50.1"},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T00:00:00Z","timestamp":1453334400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Legionnaires\u2019 disease (LD) is a globally distributed systemic infectious disease. The burden of LD in many regions is still unclear, especially in Asian countries including China. A survey of<jats:italic>Legionella<\/jats:italic>infection using real-time PCR and nested sequence-based typing (SBT) was performed in two hospitals in Shanghai, China. A total of 265 bronchoalveolar lavage fluid (BALF) specimens were collected from hospital A between January 2012 and December 2013, and 359 sputum specimens were collected from hospital B throughout 2012. A total of 71 specimens were positive for<jats:italic>Legionella<\/jats:italic>according to real-time PCR focusing on the 5S rRNA gene. Seventy of these specimens were identified as<jats:italic>Legionella pneumophila<\/jats:italic>as a result of real-time PCR amplification of the<jats:italic>dot<\/jats:italic>A gene. Results of nested SBT revealed high genetic polymorphism in these<jats:italic>L. pneumophila<\/jats:italic>and ST1 was the predominant sequence type. These data revealed that the burden of LD in China is much greater than that recognized previously, and real-time PCR may be a suitable monitoring technology for LD in large sample surveys in regions lacking the economic and technical resources to perform other methods, such as urinary antigen tests and culture methods.<\/jats:p>","DOI":"10.1017\/s0950268815003301","type":"journal-article","created":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T10:34:45Z","timestamp":1453372485000},"page":"2006-2010","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Combined use of real-time PCR and nested sequence-based typing in survey of 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