{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:19:50Z","timestamp":1790745590244,"version":"4.1.0"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T00:00:00Z","timestamp":1456963200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The Darwin region in northern Australia has experienced rapid population growth in recent years, and with it, an increased incidence of melioidosis. Previous studies in Darwin have associated the environmental presence of<jats:italic>Burkholderia pseudomallei<\/jats:italic>, the causative agent of melioidosis, with anthropogenic land usage and proximity to animals. In our study, we estimated the occurrence of<jats:italic>B. pseudomallei<\/jats:italic>and<jats:italic>Burkholderia<\/jats:italic>spp. relatives in faecal matter of wildlife, livestock and domestic animals in the Darwin region. A total of 357 faecal samples were collected and bacteria isolated through culture and direct DNA extraction after enrichment in selective media. Identification of<jats:italic>B. pseudomallei, B. ubonensis<\/jats:italic>, and other<jats:italic>Burkholderia<\/jats:italic>spp. was carried out using TTS1, Bu550, and<jats:italic>recA<\/jats:italic>BUR3\u2013BUR4 quantitative PCR assays, respectively.<jats:italic>B. pseudomallei<\/jats:italic>was detected in seven faecal samples from wallabies and a chicken.<jats:italic>B. cepacia<\/jats:italic>complex spp. and<jats:italic>Pandoraea<\/jats:italic>spp. were cultured from wallaby faecal samples, and<jats:italic>B. cenocepacia<\/jats:italic>and<jats:italic>B. cepacia<\/jats:italic>were also isolated from livestock animals. Various bacteria isolated in this study represent opportunistic human pathogens, raising the possibility that faecal shedding contributes to the expanding geographical distribution of not just<jats:italic>B. pseudomallei<\/jats:italic>but other Burkholderiaceae that can cause human disease.<\/jats:p>","DOI":"10.1017\/s0950268816000285","type":"journal-article","created":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T11:19:21Z","timestamp":1457003961000},"page":"1924-1932","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":22,"title":["The melioidosis agent<i>Burkholderia pseudomallei<\/i>and related opportunistic pathogens detected in faecal matter of wildlife and livestock in northern Australia"],"prefix":"10.1017","volume":"144","author":[{"given":"A. C. 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