{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:38:28Z","timestamp":1793813908713,"version":"4.6.1"},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T00:00:00Z","timestamp":1459123200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Healthcare workers (HCWs) reporting no history of varicella frequently receive varicella vaccination (vOka) if they test varicella-zoster virus (VZV) immunoglobulin G (IgG) negative. In this study, the utilities of VZV-IgG time-resolved fluorescence immunoassay (VZV-TRFIA) and a commercial VZV-IgG purified glycoprotein enzyme immunoassay (gpEIA) currently used in England for confirming VZV immunity have been compared to the fluorescent-antibody-to-membrane-antigen assay (FAMA). A total of 110 HCWs received two doses of vOka vaccine spaced 6 weeks apart and sera collected pre-vaccination (<jats:italic>n<\/jats:italic>= 100), at 6 weeks post-completion of vaccination (<jats:italic>n<\/jats:italic>= 86) and at 12\u201318 months follow-up (<jats:italic>n<\/jats:italic>= 73) were analysed. Pre-vaccination, by FAMA, 61\u00b70% sera were VZV IgG negative, and compared to FAMA the sensitivities of VZV-TRFIA and gpEIA were 74\u00b74% [95% confidence interval (CI) 57\u00b79\u201387\u00b70] and 46\u00b72% (95% CI 30\u00b71\u201362\u00b78), respectively. Post-completion of vaccination the seroconversion rate by FAMA was 93\u00b77% compared to rates of 95\u00b78% and 70\u00b78% determined by VZV-TRFIA and gpEIA, respectively. At 12\u201318 months follow-up seropositivity rates by FAMA, VZV-TRFIA and gpEIA were 78\u00b71%, 74\u00b70% and 47\u00b79%, respectively. Compared to FAMA the sensitivities of VZV-TRFIA and gpEIA for measuring VZV IgG following vaccination were 96\u00b74% (95% CI 91\u00b77\u201398\u00b78) and 74\u00b76% (95% CI 66\u00b75\u201381\u00b76), respectively. Using both FAMA and VZV-TRFIA to identify healthy adult VZV susceptibles and measure seroconversion showed that vOka vaccination of HCWs is highly immunogenic.<\/jats:p>","DOI":"10.1017\/s0950268816000595","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T13:17:39Z","timestamp":1459171059000},"page":"2345-2353","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":12,"title":["The differences in short- and long-term varicella-zoster virus (VZV) immunoglobulin G levels following varicella vaccination of healthcare workers measured by VZV fluorescent-antibody-to-membrane-antigen assay (FAMA), VZV time-resolved fluorescence immunoassay and a VZV purified glycoprotein enzyme immunoassay"],"prefix":"10.1017","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5534-5191","authenticated-orcid":false,"given":"P. A. 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