{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:18:28Z","timestamp":1776086308469,"version":"3.50.1"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2016,5,16]],"date-time":"2016-05-16T00:00:00Z","timestamp":1463356800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>To examine evidence of positive antibodies against immunogenic proteins of <jats:italic>Anaplasma phagocytophilum<\/jats:italic> in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific <jats:italic>Anaplasma<\/jats:italic> antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (<jats:italic>n<\/jats:italic> = 47) and from blood donors (<jats:italic>n<\/jats:italic> = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (2\u00b70%) showed positive IgM reactions and seven samples (2\u00b74%) were IgG positive for <jats:italic>A. phagocytophilum<\/jats:italic> by immunoblot. Borderline reactivity was found in 30 samples (10\u00b714%) for IgM and 36 samples (12\u00b72%) for IgG. The difference between patients and blood donors was statistically significant for IgM (<jats:italic>P<\/jats:italic> = 0\u00b7006) and for IgG (<jats:italic>P<\/jats:italic> = 0\u00b70007) antibodies. A statistically significant result was obtained for IgG (<jats:italic>P<\/jats:italic> = 0\u00b702) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to <jats:italic>A. phagocytophilum<\/jats:italic> in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.<\/jats:p>","DOI":"10.1017\/s0950268816000972","type":"journal-article","created":{"date-parts":[[2016,5,16]],"date-time":"2016-05-16T11:17:22Z","timestamp":1463397442000},"page":"2597-2604","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of antibodies against surface and outer membrane proteins of <i>Anaplasma phagocytophilum<\/i> in Lyme borreliosis and tick-borne encephalitis paediatric 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