{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:47:42Z","timestamp":1762004862473,"version":"3.37.3"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T00:00:00Z","timestamp":1491177600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A total of 46 strains of<jats:italic>Salmonella<\/jats:italic>isolated from patients with sporadic diarrhoea or involved in foodborne outbreaks were analysed by PCR for genus identification and serotyping. Subtyping was performed using pulsed-field gel electrophoresis (PFGE) and multiple amplification of phage locus typing (MAPLT) for seven variable loci. Bacteria were identified as belonging to serotype Enteritidis (33 strains; 71\u00b77%) or Typhimurium (13 strains; 28\u00b73%). A high similarity coefficient (94\u00b76%) was observed in the<jats:italic>Salmonella<\/jats:italic>Enteritidis group for which were found three related PFGE profiles and only one MAPLT; strains representing profile PA\/P1\/MI were prevalent (27; 81\u00b78%). Two<jats:italic>Salmonella<\/jats:italic>Typhimurium isolates were untypeable by PFGE. The remaining 11 strains had eight PFGE and three MAPLT profiles. The discriminatory power of MAPLT was lower than that of PFGE.<jats:italic>Salmonella<\/jats:italic>Enteritidis of clonal nature is predominant in Paran\u00e1 State, with the most prevalent profile PA\/P1\/M1 associated with sporadic diarrhoea and with seven of nine reported outbreaks. In conclusion, PFGE shows higher discriminatory power among<jats:italic>Salmonella<\/jats:italic>strains.<\/jats:p>","DOI":"10.1017\/s0950268817000619","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T10:10:53Z","timestamp":1491214253000},"page":"1953-1960","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Molecular characterisation of<i>Salmonella<\/i>strains isolated from outbreaks and sporadic cases of diarrhoea occurred in Paran\u00e1 State, South of Brazil"],"prefix":"10.1017","volume":"145","author":[{"given":"F. E. A.","family":"ASSIS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. B.","family":"DALLAGASSA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M. S. S.","family":"FARAH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"SOUZA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. O.","family":"PEDROSA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. S.","family":"CHUBATSU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0478-6544","authenticated-orcid":false,"given":"C. M. T.","family":"FADEL-PICHETH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,4,3]]},"reference":[{"key":"S0950268817000619_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.12.031"},{"key":"S0950268817000619_ref29","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-14-338"},{"volume-title":"National Salmonella Surveillance Annual Report, 2012","year":"2014","key":"S0950268817000619_ref28"},{"key":"S0950268817000619_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2008.12.002"},{"key":"S0950268817000619_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05527-11"},{"key":"S0950268817000619_ref23","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822008000300024"},{"key":"S0950268817000619_ref7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1734-1738.2004"},{"key":"S0950268817000619_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2010.09.040"},{"key":"S0950268817000619_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268802006842"},{"key":"S0950268817000619_ref41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077836"},{"key":"S0950268817000619_ref33","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1128\/JCM.33.5.1070-1074.1995","article-title":"Molecular analysis of Salmonella enteritidis by pulsed-field gel electrophoresis and ribotyping","volume":"33","author":"Thong","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268817000619_ref22","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652006000400001"},{"key":"S0950268817000619_ref19","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity","volume":"26","author":"Hunter","year":"1988","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268817000619_ref27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095976"},{"volume-title":"Surveillance for Foodborne Disease Outbreaks United States, 2013: Annual Report","year":"2015","key":"S0950268817000619_ref20"},{"key":"S0950268817000619_ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-178"},{"key":"S0950268817000619_ref21","first-page":"3991","article-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013","volume":"13","year":"2015","journal-title":"European Food Safety Authority Journal"},{"key":"S0950268817000619_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2008.08.014"},{"key":"S0950268817000619_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2012.06.008"},{"key":"S0950268817000619_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2008.05.009"},{"key":"S0950268817000619_ref3","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.20"},{"key":"S0950268817000619_ref10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02095-08"},{"key":"S0950268817000619_ref18","first-page":"523","volume-title":"Salmonella \u2013 A Diversified Superbug","author":"Ross","year":"2012"},{"key":"S0950268817000619_ref26","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0787"},{"key":"S0950268817000619_ref12","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1604-1611.2005"},{"key":"S0950268817000619_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.09.004"},{"key":"S0950268817000619_ref1","doi-asserted-by":"publisher","DOI":"10.1086\/650733"},{"key":"S0950268817000619_ref5","first-page":"654","volume-title":"Manual of Clinical Microbiology","author":"Bopp","year":"2003"},{"key":"S0950268817000619_ref2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.P11101"},{"key":"S0950268817000619_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.03.005"},{"key":"S0950268817000619_ref16","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.59"},{"key":"S0950268817000619_ref17","unstructured":"PNL05. Standard operating procedure for PulseNet PFGE of Escherichia coli O157:H7, Escherichia coli non-O157 (STEC), Salmonella serotypes, Shigella sonnei and Shigella flexneri. In: PNL05 last updated April 2013: Centers for Disease Control and Prevention (CDC), 2013."},{"key":"S0950268817000619_ref30","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822010000200033"},{"key":"S0950268817000619_ref39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02749.x"},{"key":"S0950268817000619_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00701-06"},{"key":"S0950268817000619_ref35","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762006000300010"},{"key":"S0950268817000619_ref25","doi-asserted-by":"publisher","DOI":"10.3201\/eid1908.121511"},{"key":"S0950268817000619_ref40","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805005820"},{"key":"S0950268817000619_ref14","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.4434"},{"key":"S0950268817000619_ref32","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03201-12"},{"key":"S0950268817000619_ref37","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.028597-0"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268817000619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,4]],"date-time":"2020-10-04T21:35:37Z","timestamp":1601847337000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268817000619\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,3]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["S0950268817000619"],"URL":"https:\/\/doi.org\/10.1017\/s0950268817000619","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"type":"print","value":"0950-2688"},{"type":"electronic","value":"1469-4409"}],"subject":[],"published":{"date-parts":[[2017,4,3]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}