{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:28:15Z","timestamp":1793042895373,"version":"4.5.0"},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T00:00:00Z","timestamp":1491177600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Asymptomatic dogs can be potential hosts of leptospirosis. However, the extension of this phenomenon in endemic areas has not yet been clearly defined. This study is aimed at evaluating the role of asymptomatic dogs as carriers of <jats:italic>Leptospira<\/jats:italic> in an endemic area of Brazil. A total of 131 male dogs without apparent leptospirosis symptoms were included in the study based on clinical and hematologic exams. Serum and urine samples were collected for microscopic agglutination tests (MAT) and polymerase chain reactions (PCR) targeted the <jats:italic>LipL32<\/jats:italic> gene, respectively. Forty-two dogs (32\u00b71%) presented seroreactivity (titres \u2a7e100). The serogroup Icterohaemorrhagiae was predominant, representing 92\u00b77% of the seropositive samples. Overall, leptospiral DNA was detected on 26 urine samples (19\u00b78%). PCR positivity was more common (28\u00b76%) on seropositive dogs than on seronegative (15\u00b77%) ones. Nevertheless, MAT was not correlated to PCR (<jats:italic>P<\/jats:italic> &gt; 0\u00b705). Age was not associated with seroreactivity, but dogs older than 5 years of age had 4\u00b707 more chances (odds ratio) of being carriers (PCR positive) than younger ones. Although the fact of knowing that asymptomatic dogs can act as leptospiral carriers is not new, the extension of this fact is impressive in an endemic region, and its role and impact on public health cannot be neglected.<\/jats:p>","DOI":"10.1017\/s0950268817000632","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T10:10:41Z","timestamp":1491214241000},"page":"1852-1854","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":49,"title":["High number of asymptomatic dogs as leptospiral carriers in an endemic area indicates a serious public health concern"],"prefix":"10.1017","volume":"145","author":[{"given":"R.","family":"SANT'ANNA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"VIEIRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"GRAPIGLIA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"LILENBAUM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,4,3]]},"reference":[{"key":"S0950268817000632_ref11","unstructured":"Clement J , Leptospirosis, hantavirus \u2013 Belgium: animal reservoir. ProMedmail 21 March 2014. Archive number 20140321\u00b72347100 http:\/\/www.promedmeil.org\/direct.php?id=2347100 (Accessed 02 December 2016)."},{"key":"S0950268817000632_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-013-9582-x"},{"key":"S0950268817000632_ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1991.tb00526.x"},{"key":"S0950268817000632_ref6","first-page":"237","article-title":"The panorama of animal leptospirosis in Rio de Janeiro, Brazil, regarding the seroepidemiology of the infection in tropical regions","volume":"9","author":"Martins","year":"2013","journal-title":"BioMed Central Veterinary Research"},{"key":"S0950268817000632_ref3","doi-asserted-by":"crossref","first-page":"6666","DOI":"10.3390\/ijerph110706666","article-title":"Leptospira contamination in household and environmental water in rural communities in southern Chile","volume":"11","author":"Claudia","year":"2014","journal-title":"International Journal of Environmental Research and Public Health"},{"key":"S0950268817000632_ref2","first-page":"251","article-title":"Urinary shedding of leptospires and presence of leptospira antibodies in healthy dogs from upper Bavaria","volume":"129","author":"Llewellyn","year":"2016","journal-title":"Berliner und M\u00fcnchenertier\u00e4rztlicheWochenschrift"},{"key":"S0950268817000632_ref4","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2003.222.1230"},{"key":"S0950268817000632_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0991-2"},{"key":"S0950268817000632_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"S0950268817000632_ref12","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2002.220.53"},{"key":"S0950268817000632_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132988"},{"key":"S0950268817000632_ref9","doi-asserted-by":"publisher","DOI":"10.4322\/rbcv.2014.238"},{"key":"S0950268817000632_ref7","first-page":"1","volume-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals","year":"2014"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268817000632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T20:50:22Z","timestamp":1555534222000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268817000632\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,3]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["S0950268817000632"],"URL":"https:\/\/doi.org\/10.1017\/s0950268817000632","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,3]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}