{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:10:04Z","timestamp":1789884604055,"version":"4.0.1"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T00:00:00Z","timestamp":1500508800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The burden of dengue virus infections increased globally during recent years. Though India is considered as dengue hyper-endemic country, limited data are available on disease epidemiology. The present study includes molecular characterization of dengue virus strains occurred in Hyderabad, India, during the year 2014. A total of 120 febrile cases were recruited for this study, which includes only children and 41 were serologically confirmed for dengue positive infections using non-structural (NS1) and\/or IgG\/IgM ELISA tests. RT-PCR, nucleotide sequencing and evolutionary analyses were carried out to identify the circulating serotypes\/genotypes. The data indicated a high percent of severe dengue (63%) in primary infections. Simultaneous circulation of all four serotypes and co-infections were observed for the first time in Hyderabad, India. In total, 15 patients were co-infected with more than one dengue serotype and 12 (80%) of them had severe dengue. One of the striking findings of the present study is the identification of serotype Den-1 as the first report from this region and this strain showed close relatedness to the Thailand 1980 strains but not to any of the strains reported from India until now. Phylogenetically, all four strains of the present study showed close relatedness to the strains, which are reported to be high virulent.<\/jats:p>","DOI":"10.1017\/s0950268817001479","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T09:38:25Z","timestamp":1500543505000},"page":"2563-2574","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":42,"title":["Co-circulation and co-infections of all dengue virus serotypes in Hyderabad, India 2014"],"prefix":"10.1017","volume":"145","author":[{"given":"K.","family":"VADDADI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"GANDIKOTA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. K.","family":"JAIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. S. V.","family":"PRASAD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"VENKATARAMANA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,7,20]]},"reference":[{"key":"S0950268817001479_ref39","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-55"},{"key":"S0950268817001479_ref33","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1519-1529.2006"},{"key":"S0950268817001479_ref30","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JCM.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction","volume":"30","author":"Lanciotti","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268817001479_ref26","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit594"},{"key":"S0950268817001479_ref24","first-page":"105","article-title":"Dengue hemorrhagic fever in children in Delhi","volume":"70","author":"Kabra","year":"1992","journal-title":"Bulletin of the World Health Organization"},{"key":"S0950268817001479_ref20","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268810001706"},{"key":"S0950268817001479_ref12","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S55376"},{"key":"S0950268817001479_ref3","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"S0950268817001479_ref37","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.10-0052"},{"key":"S0950268817001479_ref29","volume-title":"Dengue Guidelines for Diagnosis, Treatment, Prevention and Control","year":"2009"},{"key":"S0950268817001479_ref4","unstructured":"WHO. Dengue and Severe Dengue. Fact sheet no. 117, July 2016 (http:\/\/www.who.int\/mediacentre\/factsheets\/fs117\/en\/index.html). Accessed August 2016."},{"key":"S0950268817001479_ref35","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.61.725"},{"key":"S0950268817001479_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1348(03)00004-2"},{"key":"S0950268817001479_ref27","doi-asserted-by":"publisher","DOI":"10.2174\/1573409911666150407161535"},{"key":"S0950268817001479_ref19","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1743-422X-10-37","article-title":"Lineage shift in Indian strains of dengue virus serotype-3 (Genotype III), evidenced by detection of lineage IV strains in clinical cases from Kerala","volume":"10","author":"Anoop","year":"2013","journal-title":"Virology Journal"},{"key":"S0950268817001479_ref11","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268814002751"},{"key":"S0950268817001479_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.05.130"},{"key":"S0950268817001479_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100535"},{"key":"S0950268817001479_ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s12088-009-0057-2"},{"key":"S0950268817001479_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(99)01669-8"},{"key":"S0950268817001479_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.07.021"},{"key":"S0950268817001479_ref31","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0950268817001479_ref5","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.3390\/v3091739","article-title":"Functional RNA elements in the dengue virus genome","volume":"3","author":"Leopoldo","year":"2011","journal-title":"Viruses"},{"key":"S0950268817001479_ref14","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-5-1"},{"key":"S0950268817001479_ref13","first-page":"373","article-title":"Chaturvedi. dengue in India","volume":"136","author":"Gupta","year":"2012","journal-title":"Indian Journal of Medical Research"},{"key":"S0950268817001479_ref25","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.4269\/ajtmh.2005.73.1063","article-title":"Differences in dengue severity in infants, children, and adults in a 3-year hospital-based study in Nicaragua","volume":"73","author":"Hammond","year":"2005","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0950268817001479_ref40","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-46"},{"key":"S0950268817001479_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.10.030"},{"key":"S0950268817001479_ref2","first-page":"133","article-title":"Improved detection of dengue virus serotypes from serum samples-evaluation of single-tube multiplex RT-PCR with cell culture","volume":"30","author":"Chakravarti","year":"2006","journal-title":"Dengue Bulletin"},{"key":"S0950268817001479_ref38","doi-asserted-by":"crossref","first-page":"4738","DOI":"10.1128\/JVI.73.6.4738-4747.1999","article-title":"Dengue virus structural differences that correlate with pathogenesis","volume":"73","author":"Leitmeyer","year":"1999","journal-title":"Journal of Virology"},{"key":"S0950268817001479_ref22","unstructured":"WHO. Variable endemicity for DF\/DHF in countries of SEA region. SEARO2016. (http:\/\/www.searo.who.int\/entity\/vector_borne_tropical_diseases\/data\/data_factsheet\/en\/)."},{"key":"S0950268817001479_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2010-x"},{"key":"S0950268817001479_ref1","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","article-title":"Dengue and dengue hemorrhagic fever","volume":"3","author":"Gubler","year":"1998","journal-title":"Clinical Microbiology Reviews"},{"key":"S0950268817001479_ref16","doi-asserted-by":"publisher","DOI":"10.3201\/eid0504.990427"},{"key":"S0950268817001479_ref7","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.meegid.2009.02.003","article-title":"Molecular evolution of dengue viruses: contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease","volume":"9","author":"Scott","year":"2009","journal-title":"Infection Genetics and Evolution"},{"key":"S0950268817001479_ref8","doi-asserted-by":"crossref","first-page":"990","DOI":"10.4269\/ajtmh.2008.78.990","article-title":"Influence of the dengue serotype, previous dengue infection, and plasma viral load on clinical presentation and outcome during a dengue-2 and dengue-4 co-epidemic","volume":"78","author":"Thomas","year":"2008","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0950268817001479_ref36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0141628"},{"key":"S0950268817001479_ref15","first-page":"849","article-title":"Genetic characterization of dengue virus serotypes causing concurrent infection in an outbreak in Ernakulam, Kerala, South India","volume":"48","author":"Anoop","year":"2010","journal-title":"Indian Journal of Experimental Biology"},{"key":"S0950268817001479_ref23","doi-asserted-by":"publisher","DOI":"10.6061\/clinics\/2014(01)08"},{"key":"S0950268817001479_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-012-0756-3"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268817001479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T01:28:02Z","timestamp":1602552482000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268817001479\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,20]]},"references-count":40,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["S0950268817001479"],"URL":"https:\/\/doi.org\/10.1017\/s0950268817001479","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,20]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}