{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:43:08Z","timestamp":1792442588736,"version":"4.5.0"},"reference-count":59,"publisher":"Cambridge University Press (CUP)","issue":"15","license":[{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Little is known about<jats:italic>Salmonella<\/jats:italic>serovars circulating in backyard poultry and swine populations worldwide. Backyard production systems (BPS) that raise swine and\/or poultry are distributed across Chile, but are more heavily concentrated in central Chile, where industrialized systems are in close contact with BPS. This study aims to detect and identify circulating<jats:italic>Salmonella<\/jats:italic>serovars in poultry and swine raised in BPS. Bacteriological<jats:italic>Salmonella<\/jats:italic>isolation was carried out for 1744 samples collected from 329 BPS in central Chile. Faecal samples were taken from swine, poultry, geese, ducks, turkeys and peacocks, as well as environmental faecal samples. Confirmation of<jats:italic>Salmonella<\/jats:italic>spp. was performed using<jats:italic>invA<\/jats:italic>-polymerase chain reaction (PCR). Identification of serovars was carried out using a molecular serotyping approach, where serogroups were confirmed by a multiplex PCR of<jats:italic>Salmonella<\/jats:italic>serogroup genes for five<jats:italic>Salmonella<\/jats:italic>O antigens (i.e., D, B, C1, C2-C3, and E1), along with two PCR amplifications, followed by sequencing of<jats:italic>fliC<\/jats:italic>and<jats:italic>fljB<\/jats:italic>genes. A total of 25 samples (1\u00b74% of total samples) from 15 BPS (4\u00b76 % of total sampled BPS) were found positive for<jats:italic>Salmonella<\/jats:italic>. Positive samples were found in poultry (chickens and ducks), swine and environmental sources. Molecular prediction of serovars on<jats:italic>Salmonella<\/jats:italic>isolated showed 52\u00b70% of<jats:italic>S.<\/jats:italic>Typhimurium, 16\u00b70% of<jats:italic>S.<\/jats:italic>Infantis, 16\u00b70%<jats:italic>S.<\/jats:italic>Enteritidis, 8\u00b70%<jats:italic>S.<\/jats:italic>Hadar, 4\u00b70%<jats:italic>S.<\/jats:italic>Tennessee and 4\u00b70%<jats:italic>S.<\/jats:italic>Kentucky. Poor biosecurity measures were found on sampled BPS, where a high percentage of mixed confinement systems (72\u00b78%); and almost half of the sampled BPS with improper management of infected mortalities (e.g. selling the carcasses of infected animals for consumption). Number of birds other than chickens (<jats:italic>P<\/jats:italic>= 0\u00b7014; OR = 1\u00b704; IC (95%) = 1\u00b701\u20131\u00b707), mixed productive objective (<jats:italic>P<\/jats:italic>= 0\u00b7030; OR = 5\u00b735; IC (95%) = 1\u00b724\u201327\u00b759) and mixed animal replacement origin (<jats:italic>P<\/jats:italic>= 0017; OR = 5\u00b719; IC (95%) = 1\u00b735\u201320\u00b747) were detected as risk factors for BPS positivity to<jats:italic>Salmonella<\/jats:italic>spp. This is the first evidence of serovars of<jats:italic>Salmonella<\/jats:italic>spp. circulating in BPS from central Chile. Detected serovars have been linked to human and animal clinical outbreaks worldwide and in Chile, highlighting the importance of BPS on the control and dissemination of<jats:italic>Salmonella<\/jats:italic>serovars potentially hazardous to public health.<\/jats:p>","DOI":"10.1017\/s0950268817002175","type":"journal-article","created":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T07:05:36Z","timestamp":1505891136000},"page":"3180-3190","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":25,"title":["First detection and characterization of<i>Salmonella<\/i>spp. in poultry and swine raised in backyard production systems in central Chile"],"prefix":"10.1017","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-2499","authenticated-orcid":false,"given":"R.","family":"ALEGRIA-MORAN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"RIVERA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"TOLEDO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. I.","family":"MORENO-SWITT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"HAMILTON-WEST","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,9,20]]},"reference":[{"key":"S0950268817002175_ref20","article-title":"Using spatial tools for high impact zoonotic agents surveillance design in backyard production systems from central Chile","volume":"4","author":"Alegria-Moran","year":"2017","journal-title":"Veterinaria Mexico OA"},{"key":"S0950268817002175_ref8","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.38.7.2465-2467.2000","article-title":"Salmonella nomenclature","volume":"38","author":"Brenner","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268817002175_ref7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02137-09"},{"key":"S0950268817002175_ref42","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-16-167"},{"key":"S0950268817002175_ref32","doi-asserted-by":"publisher","DOI":"10.1079\/WPS200570"},{"key":"S0950268817002175_ref50","unstructured":"CDC. Eight Multistate Outbreaks of Human Salmonella Infections Linked to Live Poultry in Backyard Flocks (Final Update). 2016 (https:\/\/www.cdc.gov\/salmonella\/live-poultry-05-16\/). 25-10-2016."},{"key":"S0950268817002175_ref24","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1128\/JCM.33.4.802-804.1995","article-title":"Evaluation of five new plating media for isolation of Salmonella species","volume":"33","author":"Dusch","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268817002175_ref41","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.200390035"},{"key":"S0950268817002175_ref35","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2007.227.229"},{"key":"S0950268817002175_ref16","unstructured":"ISP. Bolet\u00edn Vigilancia de Laboratorio: Salmonella spp. 2012\u20132016. 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