{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T09:03:12Z","timestamp":1793782992687,"version":"4.6.1"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2018,5,6]],"date-time":"2018-05-06T00:00:00Z","timestamp":1525564800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to molecularly survey<jats:italic>Bartonella<\/jats:italic>in dogs from Chile. Quantitative real-time PCR (qPCR) for<jats:italic>Bartonella<\/jats:italic>spp. based on<jats:italic>nuoG<\/jats:italic>gene was performed in 139 blood samples taken from dogs belonging to rural localities of the Valdivia Province, Los R\u00edos region, southern Chile.<jats:italic>nuoG<\/jats:italic>qPCR-positive samples were submitted to conventional PCR assays for<jats:italic>ftsZ<\/jats:italic>,<jats:italic>gltA<\/jats:italic>,<jats:italic>rpoB<\/jats:italic>and<jats:italic>nuoG<\/jats:italic>genes and sequencing for speciation and phylogenetic analysis. Based upon qPCR results,<jats:italic>Bartonella<\/jats:italic>spp. occurrence in dogs was 4.3% (6\/139). Out of six<jats:italic>nuoG<\/jats:italic>qPCR-positive samples, six, three, two and none showed positive results in cPCR assays based on<jats:italic>gltA<\/jats:italic>,<jats:italic>ftsZ<\/jats:italic>,<jats:italic>rpoB<\/jats:italic>and<jats:italic>nuoG<\/jats:italic>genes, respectively. Consistent sequencing results were obtained only for the<jats:italic>ftsZ<\/jats:italic>gene from sample #1532 (GeneBank accession number: MG252491), and<jats:italic>gltA<\/jats:italic>gene from samples #1535 (MG252490) and #1532 (148 bp fragment that was not deposited in GenBank). Phylogenetic analysis of<jats:italic>ftsZ<\/jats:italic>and<jats:italic>gltA<\/jats:italic>genes allowed speciation of two<jats:italic>nuoG<\/jats:italic>-positive samples, one as<jats:italic>Bartonella vinsonii<\/jats:italic>subsp. berkhoffii and the other as<jats:italic>B. henselae. Bartonella vinsonii<\/jats:italic>subsp. berkhoffii and<jats:italic>B. henselae<\/jats:italic>are detected for the first time in dogs from Chile, highlighting the importance of the canine population as a source of zoonotic agents and potential infection risk to humans.<\/jats:p>","DOI":"10.1017\/s0950268818001127","type":"journal-article","created":{"date-parts":[[2018,5,6]],"date-time":"2018-05-06T02:43:55Z","timestamp":1525574635000},"page":"1202-1204","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":23,"title":["<i>Bartonella vinsonii subsp<\/i>. berkhoffii and<i>B<\/i>.<i>henselae<\/i>in dogs"],"prefix":"10.1017","volume":"146","author":[{"given":"A.","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sep\u00falveda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Bittencourt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Benevenute","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Z.","family":"Machado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2018,5,6]]},"reference":[{"key":"S0950268818001127_ref13","doi-asserted-by":"publisher","DOI":"10.4067\/S0034-98872005001200008"},{"key":"S0950268818001127_ref12","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X15593787"},{"key":"S0950268818001127_ref11","first-page":"7154","volume-title":"Applied Environmental Microbiology","volume":"15","author":"Gon\u00e7alves","year":"2016"},{"key":"S0950268818001127_ref9","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2007023"},{"key":"S0950268818001127_ref8","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812000519"},{"key":"S0950268818001127_ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-010-9735-1"},{"key":"S0950268818001127_ref5","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X15593787"},{"key":"S0950268818001127_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.06.001"},{"key":"S0950268818001127_ref2","first-page":"1","article-title":"Prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile","volume":"12","author":"M\u00fcller","year":"2016","journal-title":"Parasitology"},{"key":"S0950268818001127_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.01.010"},{"key":"S0950268818001127_ref1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050931"},{"key":"S0950268818001127_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.12.003"},{"key":"S0950268818001127_ref14","doi-asserted-by":"publisher","DOI":"10.3201\/eid1306.061337"},{"key":"S0950268818001127_ref7","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1128\/JCM.33.7.1797-1803.1995","article-title":"Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene","volume":"33","author":"Norman","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"S0950268818001127_ref3","doi-asserted-by":"publisher","DOI":"10.3201\/eid1507.081570"}],"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268818001127","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T16:45:57Z","timestamp":1604249157000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268818001127\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,6]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["S0950268818001127"],"URL":"https:\/\/doi.org\/10.1017\/s0950268818001127","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,6]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2018\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}