{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T02:18:50Z","timestamp":1780366730067,"version":"3.54.1"},"reference-count":47,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"unspecified","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100012095","name":"Scottish Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012095","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p><jats:italic>Klebsiella pneumoniae<\/jats:italic> are opportunistic pathogens which can cause mastitis in dairy cattle. <jats:italic>K. pneumoniae<\/jats:italic> mastitis often has a poor cure rate and can lead to the development of chronic infection, which has an impact on both health and production. However, there are few studies which aim to fully characterize <jats:italic>K. pneumoniae<\/jats:italic> by whole-genome sequencing from bovine mastitis cases. Here, <jats:italic>K. pneumoniae<\/jats:italic> isolates associated with mastitis in dairy cattle were identified using matrix-assisted laser desorption\/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and whole-genome sequencing. Furthermore, whole-genome sequence data were used for phylogenetic analyses and both virulence and antimicrobial resistance (AMR) prediction, in parallel with phenotypic AMR testing. Forty-two isolates identified as <jats:italic>K. pneumoniae<\/jats:italic> were subject to whole-genome sequencing, with 31 multi-locus sequence types being observed, suggesting the source of these isolates was likely environmental. Isolates were examined for key virulence determinants encoding acquired siderophores, colibactin, and hypermucoidy. The majority of these were absent, except for <jats:italic>ybST<\/jats:italic> (encoding yersiniabactin) which was present in six isolates. Across the dataset, there were notable levels of phenotypic AMR against streptomycin (26.2%) and tetracycline (19%), and intermediate susceptibility to cephalexin (26.2%) and neomycin (21.4%). Of importance was the detection of two ESBL-producing isolates, which demonstrated multi-drug resistance to amoxicillin-clavulanic acid, streptomycin, tetracycline, cefotaxime, cephalexin, and cefquinome.<\/jats:p>","DOI":"10.1017\/s0950268824001754","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T08:47:40Z","timestamp":1737103660000},"update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":12,"title":["Antimicrobial resistance profiles and molecular epidemiology of <i>Klebsiella pneumoniae<\/i> isolates from Scottish bovine mastitis cases"],"prefix":"10.1017","volume":"153","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2260-592X","authenticated-orcid":false,"given":"Jolinda","family":"Pollock","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Foster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina","family":"Henderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Hutchings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin K.","family":"Paterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"key":"S0950268824001754_r46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0293477"},{"key":"S0950268824001754_r30","unstructured":"[30] CLSI, Pennsylvania, United States (2023) CLSI Vet 01S: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, 6th Edn."},{"key":"S0950268824001754_r12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2017.11.021"},{"key":"S0950268824001754_r4","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02997-22"},{"key":"S0950268824001754_r20","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000162"},{"key":"S0950268824001754_r26","first-page":"12","article-title":"A genomic surveillance framework and genotyping tool for Klebsiella pneumoniae and its related species complex","author":"Lam","year":"2021","journal-title":"Nature Communications"},{"key":"S0950268824001754_r5","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2012-6078"},{"key":"S0950268824001754_r35","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15721"},{"key":"S0950268824001754_r23","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"S0950268824001754_r17","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00752-13"},{"key":"S0950268824001754_r28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz239"},{"key":"S0950268824001754_r31","unstructured":"[31] CASFM, Paris, France (2023) Comit\u00e9 de l\u2019antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie."},{"key":"S0950268824001754_r32","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"S0950268824001754_r29","unstructured":"[29] EUCAST (2023) The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. 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Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}],"article-number":"e15"}}