{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T02:44:11Z","timestamp":1766544251599},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1997,4,1]],"date-time":"1997-04-01T00:00:00Z","timestamp":859852800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1997,4]]},"abstract":"<jats:p>Rapid, non-culture, serogroup determination of meningococcal infection\n \n\nis important in contact \n\nmanagement where vaccination may be possible. The impending availability\n of \n\npolysaccharide\u2013protein conjugate vaccines for serogroup C disease\n requires \n\nmaximal case \n\nascertainment, with serogroup determination, at a time when the number\n of \n\nculture confirmed \n\nmeningococcal infections is decreasing. A polymerase chain reaction assay\n (PCR), \n\nbased on a \n\nrestriction fragment length polymorphism (RFLP) in the meningococcal serogroup\n B \n\nand C \n\nsialyltransferase (<jats:italic>siaD<\/jats:italic>) gene, was developed to combine the non-culture\n \n\ndiagnosis of \n\nmeningococcal infection from CSF, whole blood and serum with serogroup\n (B and C) \n\nidentification. The PCR assay was adapted to an ELISA format incorporating\n \n\nhybridization \n\nwith serogroup-specific B and C oligonucleotide probes. Specificity for\n CSFs \n\nwas 100% and \n\nsensitivities were respectively 81, 63 and 30% for CSFs, whole blood and\n sera. \n\nThe serogroup-specific PCR ELISA is a significant addition to currently\n \n\navailable tests for non-culture \n\ndiagnosis of meningococcal infection and outbreak investigation.<\/jats:p>","DOI":"10.1017\/s0950268896007261","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:23:42Z","timestamp":1027776222000},"page":"111-117","source":"Crossref","is-referenced-by-count":96,"title":["Non-culture diagnosis and serogroup determination of meningococcal\n B \n\nand C infection by a sialyltransferase (<i>siaD<\/i>) PCR ELISA"],"prefix":"10.1017","volume":"118","author":[{"given":"R.","family":"BORROW","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"CLAUS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"GUIVER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"SMART","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M.","family":"JONES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. B.","family":"KACZMARSKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"FROSCH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"FOX","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,4,1]]},"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268896007261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,7]],"date-time":"2019-04-07T19:59:04Z","timestamp":1554667144000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268896007261\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,4]]}},"alternative-id":["S0950268896007261"],"URL":"https:\/\/doi.org\/10.1017\/s0950268896007261","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,4]]}}}