{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T21:47:16Z","timestamp":1766440036675},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1997,10,1]],"date-time":"1997-10-01T00:00:00Z","timestamp":875664000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>Three members of family A, who had diarrhoea on 20 October, lived\n on \n\na small arable farm \n\nwhich had 10 cattle. Manure from the animals was used to fertilize the\n \n\nground for growing \n\npotatoes which were then offered for retail sale, unwashed, directly from\n \n\nthe farm. The mother \n\nfrom family B bought potatoes, which were covered with manure, from \n\nfamily A in early \n\nNovember and over the subsequent 10 days she became ill with diarrhoea\n and \n\nher daughter and son both became ill with bloody diarrhoea. The mother\n from family C \n\nvisited family B \n\nwhile the daughter from the latter family was symptomatic; the mother \n\ndeveloped diarrhoea \n\nseveral days later. The mother and two sons from family D visited family\n \n\nB while the son from \n\nthe latter family was symptomatic; the first son developed bloody diarrhoea\n \n\n6 days later which \n\nprogressed to development of haemolytic-uraemic syndrome. Direct culture\n \n\nof faecal samples \n\nonto cefixime rhamnose sorbitol MacConkey agar failed to isolate <jats:italic>E.\n \n\ncoli<\/jats:italic> O157 from any of \n\nthe symptomatic patients, and direct culture onto cefixime tellurite \n\nsorbitol MacConkey agar \n\nisolated the organism from only one patient. In contrast, a combination\n of \n\nisolation of <jats:italic>E. coli<\/jats:italic> \n\nO157 by immunomagnetic separation and detection of <jats:italic>E. coli<\/jats:italic> \n\nO157-specific secretory IgA, \n\nsuggested <jats:italic>E. coli<\/jats:italic> O157 infection in all eight symptomatic patients,\n \n\nbut not in any of the family \n\nmembers who were not ill. Two children who excreted the organism for 60\n and 89 days \n\nrespectively were the only two patients who did not develop a secretory\n \n\nIgA response. <jats:italic>E. coli<\/jats:italic> \n\nO157 was not isolated from potatoes from the farm and faecal samples from\n \n\nthe farm animals were not available for examination. The study illustrates\n \n\nthe need to use the most sensitive \n\nmethods available during the investigation and follow up of cases of \n\n<jats:italic>E. coli<\/jats:italic> O157 infection.<\/jats:p>","DOI":"10.1017\/s0950268897007991","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:23:42Z","timestamp":1027761822000},"page":"113-119","source":"Crossref","is-referenced-by-count":30,"title":["An outbreak of infection due to verocytotoxin-producing \n\n<i>Escherichia coli<\/i> O157 in four families: the influence of \n\nlaboratory methods on the outcome of the investigation"],"prefix":"10.1017","volume":"119","author":[{"given":"P. A.","family":"CHAPMAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. A.","family":"SIDDONS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"MANNING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"CHEETHAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,10,1]]},"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268897007991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T16:07:44Z","timestamp":1557590864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268897007991\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,10]]}},"alternative-id":["S0950268897007991"],"URL":"https:\/\/doi.org\/10.1017\/s0950268897007991","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10]]}}}