{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:49:03Z","timestamp":1759009743567},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epidemiol. Infect."],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p>Since 1992, the proportion of culture-confirmed meningococcal infections\n compared with \nnumbers of notified cases of meningococcal disease has decreased in England\n and Wales. As \nmost meningococcal strain characterization methods depend on a clinical\n isolate, this has \nresulted in a loss of epidemiological information. To address this problem,\n and to aid \nnon-culture diagnosis, a semi-nested PCR protocol for the amplification\n \nof the meningococcal <jats:italic>porB<\/jats:italic> \ngene from clinical specimens was developed. This gene encodes the meningococcal\n serotyping \nantigen; strain typing data was provided by hybridization of allele-specific\n oligonucleotide \nprobes to the digoxigenin-labelled <jats:italic>porB<\/jats:italic> amplicon in a \n\u2018PCR ELISA\u2019. This assay was specific \nfor meningococcal DNA and sensitivities of 0\u00b781 for cerebrospinal\n \nfluid (CSF), 0\u00b757 for serum, \nand 0\u00b762 for whole blood taken from patients with proven meningococcal\n infection were \nobtained.<\/jats:p>","DOI":"10.1017\/s0950268898008656","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:23:42Z","timestamp":1027776222000},"page":"257-262","source":"Crossref","is-referenced-by-count":27,"title":["Amplification of the meningococcal <i>porB<\/i> gene for non-culture\n \nserotype characterization"],"prefix":"10.1017","volume":"120","author":[{"given":"R.","family":"URWIN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. B.","family":"KACZMARSKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"GUIVER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"FOX","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C. J.","family":"MAIDEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,6,1]]},"container-title":["Epidemiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0950268898008656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T21:04:58Z","timestamp":1554239098000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0950268898008656\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["S0950268898008656"],"URL":"https:\/\/doi.org\/10.1017\/s0950268898008656","relation":{},"ISSN":["0950-2688","1469-4409"],"issn-type":[{"value":"0950-2688","type":"print"},{"value":"1469-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6]]}}}